Abstract
Since DNA damages of different types are subjected to cellular recombination repair, recombinationless bacteria are usually more sensitive than wild ones. Agents showing increased lethal activity on Rec− over Rec+ cells may have damaged DNA. The rec- assay (1) is extremely simple and inexpensive, and positive chemicals can easily be pooled. These samples should be examined for their capacity to induce mutations in bacteria. Bacillus subtilis is profitable in the assay of chemicals because its cellular membrane is more permeable to chemicals than enteric bacteria. A number of new environmental mutagens among food additives (1–3) (such as phloxine and AF2), pesticides (4–6) (such as Dexon and Vamidothion), or heavy metals (7,8) (chromium and vanadium) have been detected as a result of the isolation and use of radiation supersensitive strains (9).
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© 1981 Plenum Press, New York
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Kada, T. (1981). Mutagenicity of Selected Chemicals in the Rec-Assay in Bacillus Subtilis . In: De Serres, F.J., Shelby, M.D. (eds) Comparative Chemical Mutagenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3409-5_3
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DOI: https://doi.org/10.1007/978-1-4613-3409-5_3
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