Mutagenicity of Selected Chemicals in the Mammalian Spot Test

  • R. Fahrig
  • G. W. P. Dawson
  • L. B. Russell

Abstract

The spot test is an in vivo method for the detection of genetic alterations, including point mutations, in somatic cells of mice. Mouse embryos which are heterozygous for different recessive coat-color genes are treated in utero at one or more stages between days 7 and 11 postconception by injection of a mutagen into the peritoneal cavity of the mother animal or by other appropriate routes of administration. If this treatment leads in a pigment precursor cell to an alteration of the wild-type allele of one of the genes under study or to its loss, a color spot in the adult coat may be seen. As each mouse observed represents many treated embryonic cells in which a mutation could have occurred, and as the whole spectrum of chromosome damage and DNA-alterations should be covered, this method is sensitive to mutagens of all types of action.

Keywords

Migration Ethyl Peri Cyclophosphamide Mitomycin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Russell, L. B., and Major, M. H. Genetics 42: 161–175 (1957).Google Scholar
  2. 2.
    Fahrig, R. Molec. Gen. Genet. 138: 309–314 (1975).CrossRefGoogle Scholar
  3. 3.
    Davidson, G. E., and Dawson, G. W. P. Mutat. Res. 38: 151–154 (1976).Google Scholar
  4. 4.
    Neuhäuser, A. GSF-Bericht B 798, München, 1977, pp. 42–43.Google Scholar
  5. 5.
    Fahrig, R., and Seiler, J. P. In preparation.Google Scholar
  6. 6.
    Russell, L. B. Arch. Toxicol. 38: 75–85 (1977).CrossRefGoogle Scholar
  7. 7.
    Fahrig, R. Arch. Toxicol. 38: 87–98 (1977).CrossRefGoogle Scholar
  8. 8.
    Carter, J., and Dawson, G. W. P. The establishment of a convenient test for somatic mutation in mammals. Progress Report, Jan. 1977-June 1977, Contract 169-77-1 ENV-EIR of the EEC Environmental Research Programme.Google Scholar
  9. 9.
    Dawson, G. W. P. Personal communication.Google Scholar
  10. 10.
    Dawson, G. W. P., Davidson, G. E., and Carter, J. The establishment of a convenient test for somatic mutation in mammals. Progress Report April - December 1976, Contract 023-74-ENV-EIR (G. W. P., Dawson) of the EEC Environmental Research Programme.Google Scholar
  11. 11.
    Fahrig, R. In: Chemical Mutagens, Principles and Methods for their Detection, Vol. 5, A. Hollaender and F. J. de Serres, Eds., Plenum Press, New York, 1978, pp. 151–176.Google Scholar
  12. 12.
    Russell, L. B. Personal communication.Google Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • R. Fahrig
    • 1
  • G. W. P. Dawson
    • 2
  • L. B. Russell
    • 3
  1. 1.SchöneckstrasseGreigurgFederal Republic of Germany
  2. 2.Department of GeneticsTrinity CollegeDublin, EireIreland
  3. 3.Oak Ridge National LaboratoryBiology DivisionOak RidgeUSA

Personalised recommendations