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Mutagenicity of Selected Chemicals in the Host-Mediated Assay

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Comparative Chemical Mutagenesis

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Abstract

Each test for mutagenicity comprises two components, a metabolic activation and a response system. An effective metabolizing system is especially necessary, since the majority of mutagens are promutagens and require biotransformation to the reactive metabolites or ultimate mutagens. The development of well characterized and highly sensitive genetic indicators (bacteriophages, bacteria, yeasts, fungi, mammalian cell cultures) which themselves do not possess the capacity for metabolic activation as do living mammals, led to the development of two-component tests later called by Garbridge and Legator host-mediated assay. The introduction of foreign mammalian cells into laboratory animals for mutagenicity testing was established about 20 years ago, tumors being used as genetic indicators for the detection of chromosomal damage. Ten years later, this method was extended to the use of microbial indicators and mammalian cell cultures. Today, practically all types of genetic changes may be detected, depending on the indicator component used, such as gene mutations, gene conversions, and recombinations.

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Author information

Authors and Affiliations

  1. Zentral Institut für Genetik und Kulturpflanzenforschung, Akademie der Wissenschaften der DDR, 4325, Gatersleben, Ost-Deutschland

    R. Braun & J. Schöneich

  2. Department of Preventive Medicine and Community Health, University of Texas Medical Branch, Galveston, Texas, 77550, USA

    M. S. Legator

  3. Inveresk Research International Musselburgh, Midlothian, EH21 7UB, UK

    D. B. McGregor

  4. Department of Genetics and Chemical Mutagenesis, Wassenaarsweg 72, State University of Leiden, Leiden, The Netherlands

    G. R. Mohn

Authors
  1. R. Braun
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  2. J. Schöneich
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  3. M. S. Legator
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  4. D. B. McGregor
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  5. G. R. Mohn
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Editor information

Editors and Affiliations

  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. De Serres  & Michael D. Shelby  & 

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© 1981 Plenum Press, New York

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Braun, R., Schöneich, J., Legator, M.S., McGregor, D.B., Mohn, G.R. (1981). Mutagenicity of Selected Chemicals in the Host-Mediated Assay. In: De Serres, F.J., Shelby, M.D. (eds) Comparative Chemical Mutagenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3409-5_15

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  • DOI: https://doi.org/10.1007/978-1-4613-3409-5_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3411-8

  • Online ISBN: 978-1-4613-3409-5

  • eBook Packages: Springer Book Archive

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