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ESR Studies of Calcium- and Proton-Induced Phase Separations in Phosphatidylserine—Phosphatidylcholine Mixed Membranes

  • Shun-ichi Ohnishi
  • Satoru Tokutomi

Abstract

A wealth of information on biological systems has been obtained by spin labels based on the sensitive response of the electron spin resonance spectrum to the rate and mode of rotational motions of the labels. The technique has been used to detect changes in the local environments of spin labels attached to biomolecules, which reflect conformational changes of the biomolecules. Spin-labeled lipids give detailed and useful information on anisotropic motions in model as well as biological membranes. Flexibility of the lipid alkyl chains can be easily measured and used as a convenient measure of the “fluidity” of membranes. When spin labels are attached rigidly to biomolecules, the spin label motion directly represents rotational motion of the biomolecules as a whole. Saturation transfer ESR spectroscopy is powerful in this area of study since it extends the time limit of sensitive detection of rotational motions to 10−3 sec (see, for example, Berliner, 1976, 1979).

Keywords

Mole Fraction Phase Separation Spin Label Induce Phase Separation Lateral Phase Separation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Shun-ichi Ohnishi
    • 1
  • Satoru Tokutomi
    • 1
  1. 1.Department of Biophysics, Faculty of ScienceKyoto UniversityKyoto 606Japan

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