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Methods for Delivering Tracers

  • George F. Alheid
  • Stephen B. Edwards
  • Stephen T. Kitai
  • Melburn R. Park
  • Robert C. SwitzerIII

Abstract

The delivery of chemical tracers into the central nervous system is often an essential, although sometimes problematic aspect of modern tract-tracing experiments. In this chapter we shall concentrate on the most common methods of tracer delivery: small injections of a solution, either using a microsyringe or through glass micropipettes, or extracellular of intracellular iontophoresis. It should be kept in mind, however, that other methods of delivering tracers may be more suitable for certain types of problems. Tracers have been injected into the periphery—for instance, into muscle in order to label motoneurons in the spinal cord or brain stem (Kristenson, 1970; Kristenson et al., 1971; Kristenson and Olsson, 1973)—and they have been applied to the cut or crushed ends of peripheral nerves (Kristenson, 1975; lies and Mulloney, 1971; Tweedle, 1978) and deposited in naturally closed structures such as the eye (LaVail and LaVail, 1972, 1974). In cases where diffusion from the site of deposition is not a serious limitation, tracers may be applied directly to the surface of the brain, either as a liquid or by means of absorbant pellets or wicks that have been soaked in the tracer solution (Arbuthnott, 1969; Descarries and Lapierre, 1973; Held and Young, 1969; Weiss and Holland, 1967).

Keywords

Current Source Pressure Injection Cocoa Butter Glass Micropipette Tracer Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • George F. Alheid
    • 1
  • Stephen B. Edwards
    • 2
  • Stephen T. Kitai
    • 3
  • Melburn R. Park
    • 3
  • Robert C. SwitzerIII
    • 4
  1. 1.Department of NeurologyUniversity of Virginia School of MedicineCharlottesvilleUSA
  2. 2.Department of AnatomyUniversity of Virginia School of MedicineCharlottesvilleUSA
  3. 3.Department of AnatomyMichigan State UniversityEast LansingUSA
  4. 4.Comparative Animal Research LaboratoryOak RidgeUSA

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