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The 2-Deoxyglucose Method

  • Chapter
Neuroanatomical Tract-Tracing Methods

Abstract

The [14C]-2-deoxy-D-glucose (2DG) method, developed by Sokoloff et al. (1977) for the purpose of studying local cerebral glucose metabolism in vivo, can provide information concerning the functional organization of larger arrays of neurons. For example, it is possible to examine functional connectivity of an entire sensory pathway, such as that of the somesthetic system, within an individual animal (e.g., Hand et al., 1977; Fig. 1). Such mapping is made possible because of the close link between brain metabolism, which in the adult is almost entirely dependent upon glucose, and neural function (Sokoloff et al., 1977; Sokoloff, 1977). For example, recent work of Yarowsky et al. (1979) and Miyaoka et al. (1979) has demonstrated a linear relationship between the amount of peripheral stimulation and the values of increased glucose metabolism in such structures as the superior cervical ganglion following electrical stimulation of its afferents and the dorsal lateral geniculate nucleus following photic stimulation of the retina.

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Author information

Authors and Affiliations

  1. Laboratories of Anatomy, Department of Animal Biology, School of Veterinary Medicine, and Institute of Neurological Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA

    Peter J. Hand

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  1. Peter J. Hand
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Editors and Affiliations

  1. Department of Neurology, University of Virginia School of Medicine, Charlottesville, Virginia, 22908, USA

    Lennart Heimer

  2. University of California, Irvine, California, USA

    Martine J. Robards

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© 1981 Plenum Press

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Hand, P.J. (1981). The 2-Deoxyglucose Method. In: Heimer, L., Robards, M.J. (eds) Neuroanatomical Tract-Tracing Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3189-6_15

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  • DOI: https://doi.org/10.1007/978-1-4613-3189-6_15

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-3191-9

  • Online ISBN: 978-1-4613-3189-6

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