Abstract
The unique contribution of the Golgi methods is their well-known ability to demonstrate soma, axons, and dendrites together and thereby to permit classification of neurons at the light microscopic level. Chapter 9 deals with various aspects of this subject. With the aid of electron microscopy (EM), on the other hand, details of synaptology and other ultrastructural features can be demonstrated. A combined Golgi-EM strategy makes possible ultrastructural analysis of synaptic relations where one of the components of a synapse is classified by means of Golgi impregnation. The impregnated component may be either post- or presynaptic. A few such situations suitable for Golgi-EM analysis will be mentioned (see Fig. 1).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Blackstad, T. W., 1965, Mapping of experimental axon degeneration by electron microscopy of Golgi preparations, Z. Zellfarsch. 67: 819–834.
Blackstad, T. W., 1969, Studies on the hippocampus: Methods of analysis, in: The Interneuron ( M. Brazier, ed.), pp. 391–414, UCLA Forum in Medical Sciences, Los Angeles.
Blackstad, T. W., 1970, Electron microscopy of Golgi preparations for the study of neuronal relations, in: Contemporary Research Methods in Neuroanatomy ( W. J. H. Nauta and S. O. E. Ebbeson, eds.), pp. 186–216, Springer-Verlag, Berlin.
Blackstad, T. W., 1975a, Golgi preparations for electron microscopy: Controlled reduction of the silver chromate by ultraviolet illumination, in: Golgi Centennial Symposium, Perspectives in Neurobiology ( M. Santini, ed.), pp. 123–132, Raven Press, New York.
Blackstad, T. W., 1975b, Electron microscopy of experimental axonal degeneration in photo-chemically modified Golgi preparations: A procedure for precise mapping of nervous connections, Brain Res. 95: 191–210.
Fairén, A., and Valverde, F., 1980, A specialized type of neuron in the visual Cortex of cat: a Golgi and electron miscroscope study of chandelier cells, J. Comp. Neurol 194: 761–779.
Fairén, A., Peters, A., and Saldanha, J., 1977, A new procedure for examining Golgi impregnated neurons by light and electron microscopy, J. Neurocytol. 6: 311–337.
Heimer, L., 1970, Bridging the gap between light and electron microscopy in the experimental tracing of fiber connections, in: Contemporary Research Methods in Neuroanatmiy ( W. H. J. Nauta and S. O. E. Ebbeson, eds.), pp. 162–172, Springer-Verlag, Berlin.
Holländer, H., 1970, The section embedding (SE) technique. A new method for the combined light microscopic and electron microscopic examination of central nervous tissue, Brain Res. 20: 39–47.
LeVay, S., 1973, Synaptic patterns in the visual cortex of the cat and monkey. Electron microscopy of Golgi preparations, J. Camp. Neurol. 150: 53–86.
Obregia, A., 1890, Fixirungsmethode der Golgi’schen Präparate des centralen Nervensystems, Arch. Pathol. Anat. 122: 387–388.
Peters, A., and Fairen, A., 1978, Smooth and sparsely-spined stellate cells in the visual cortex of the rat: A study using a combined Golgi-electron microscope technique, J. Cmnp. Neurol. 181: 129–172.
Peters, A., Proskauer, C. C., Feldman, M. L., and Kimerer, L., 1979, The projection of the lateral geniculate nucleus to area 17 of the rat cerebral cortex. V. Degenerating axon terminals synapsing with Golgi impregnated neurons, J. Neurocytol. 8: 331–357.
Pinching, A. J., and Brooke, R. N. L., 1973, Electron microscopy of single cells in the olfactory bulb using Golgi impregnation, J. Neurocytol. 2: 157–170.
Ramon-Moliner, E., and Ferrari, J., 1972, Electron microscopy of previously identified cells and processes within the central nervous system, J Neurocytol. 1: 85–100.
Ramon-Moliner, E., and Ferrari, J., 1976, Electron microscopy of Golgi-stained material following lead Chromate substitution, Brain. Res. 103: 339–344.
Ribi, W. A., 1976, A Golgi-electron microscope method for insect nervous tissue. Stain Technol. 51: 13–16.
Scott, G. L., and Guillery, R. W., 1974, Studies with the high voltage electron microscope of normal, degenerating, and Golgi impregnated neuronal processes, J. Neurocytol. 3: 567–590.
Somogyi, P., 1978, The study of Golgi stained cells and of experimental degeneration under the electron microscope: A direct method for the identification in the visual cortex of three successive links in a neuron chain, Neuroscience 3: 167–180.
Somogyi, P., and Cowey, A., 1981, Combined Golgi and electron microscopic study on the synapses formed by double bouquet cells in the visual cortex of the cat and monkey. J. Comp. Neurol. 195: 547–566.
Somogyi, P., and Smith, A. D., 1979, Projection of neostriatal spiny neurons to the substantia nigra. Application of a combined Golgi-staining and horseradish peroxidase transport procedure at both light and electron microscope levels, Brain Res. 178: 3–15.
Somogyi, P., Hodgson, A. F. and Smith, A. D., 1979, An approach to tracing neuron networks in the cerebral cortex and basal ganglia. Combination of Golgi staining, retrograde transport of horseradish peroxidase and arterograde degeneration of synaptic boutons in the same material, Neuroscience 4: 1804–1852.
Somogyi, P., Bolam, F. P., and Smith, A. D., 1981, Monosynaptic cortical input and local axon collaterals of identified striate-nigral neurons. A light and electron microscopic study using the Golgi-peroxidase transport-degeneration procedure, J. Comp. Neurol. 195: 567–584.
Stell, W. K., 1965, Correlation of retinal cytoarchitecture and ultrastructure in Golgi preparations, Anat. Rec. 153: 389–397.
Stell, W. K., 1967, The structure and relationships of horizontal cells and photoreceptor-bipolar synaptic complexes in goldfish retina, Am. J. Anat. 121: 401–424.
Stell, W. K., and Witkovsky, P., 1973, Retinal structure in the smooth dogfish, Mustelus canis: General description and light microscopy of giant ganglion cells, J. Comp. Neurol. 148: 1–31.
Stell, W. K., Lightfoot, D. O., Wheeler, T. G., and Leeper, H. F., 1975, Goldfish retina: Functional polarization of some horizontal cell dendrites and synapses. Science 190: 989–990.
Valverde, F., 1976, Aspects of cortical organization related to the geometry of neurons with intra-cortical axons, J. Neurocytol. 5: 509–529.
White, E. L., 1978, Identified neurons in mouse SmI cortex which are postsynaptic to thalamocortical axon terminals: A combined Golgi-electron microscopic and degeneration study, J. Comp. Neurol. 181: 627–661.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1981 Plenum Press
About this chapter
Cite this chapter
Blackstad, T.W. (1981). Tract Tracing by Electron Microscopy of Golgi Preparations. In: Heimer, L., Robards, M.J. (eds) Neuroanatomical Tract-Tracing Methods. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3189-6_12
Download citation
DOI: https://doi.org/10.1007/978-1-4613-3189-6_12
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-3191-9
Online ISBN: 978-1-4613-3189-6
eBook Packages: Springer Book Archive