Use of Concanavalin A to Monitor Changes in Glycoprotein Synthesis during Early Mouse Development

  • Terry Magnuson
  • Charles J. Epstein

Abstract

One- and two-dimensional gel electrophoresis have been used to examine proteins synthesized by preimplantation mouse embryos and, for each stage analyzed, about 400–600 distinct polypeptides have been resolved (Epstein and Smith, 1974, Van Blerkom and Brockway, 1975, Levinson et al., 1978). In an attempt to characterize further these proteins, we have used concanavalin A (Con A), a plant lectin that binds to mannose residues, to precipitate both total and cell surface glycoproteins synthesized during the preimplantation period. The precipitates were subsequently analyzed by two-dimensional gel electrophoresis and autoradiography.

Keywords

Electrophoresis Polypeptide Methionine Galactose Staphylococcus 

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References

  1. Epstein, C. J., and Smith, S. A., 1974, Electrophoretic analysis of proteins synthesized by preimplantation mouse embryos. Dev. Biol. 40: 233–244.CrossRefGoogle Scholar
  2. Johnson, L. V., and Calarco, P. G., 1980, Mammalian preimplantation development: The cell surface. Anat Rec. 196: 201–219.CrossRefGoogle Scholar
  3. Levinson, J., Goodfellow, P., Vadeboncoeur, M., and McDevitt, H., 1978, Identification of stage-specific polypeptides synthesized during murine preimplantation development, Proc. Natl. Acad. Set. USA 75: 3332–3336.CrossRefADSGoogle Scholar
  4. Van Blerkom, J., and Brockway, G. O., 1975, Qualitative patterns of protein synthesis in the preimplantation mouse embryo. I. Normal pregnancy. Dev. Biol. 44: 148–157.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Terry Magnuson
    • 1
    • 2
  • Charles J. Epstein
    • 1
    • 2
  1. 1.Department of PediatricsUniversity of CaliforniaSan FranciscoUSA
  2. 2.Department of Biochemistry and BiophysicsUniversity of CaliforniaSan FranciscoUSA

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