Lysosomal Mechanisms in Blastocyst Implantation and Early Decidualization

  • Bruce C. Moulton
  • Sudha Elangovan


The lysosome with its diverse content of hydrolytic enzymes has the capacity to degrade virtually every macromolecule of biological origin. Precise control of this self-destructive potential provides eukaryotic cells with the means for intracellular digestion and the means for adaptation to changes in nutritional and hormonal variables. Intracellular digestion of exogenous material taken into cells by pinocytosis or phagocytosis provides substrate for the synthesis of new cellular organelles and enzymes. Endogenous macromolecules and pieces of cytoplasmic material sequestered and digested by lysosomes during autophagia enable redirected physiological function as cellular components are degraded and resynthe sized. These lysosomal mechanisms appear to be involved in the pinocytosis of the uterine lumenal epithelium during the preimplantation period of pregnancy, the penetration of the lumenal epithelium by the blastocyst, and the extensive remodeling of the endometrial stroma during decidualization.


Lysosomal Enzyme Implantation Site Decidual Cell Uterine Epithelium Mouse Uterus 
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  1. Abraham, R., Hendy, R., Dougherty, W. J., Fulfs, J. C., and Golberg, L., 1970, Participation of lysosomes in eariy implantation in the rabbit, Exp. Mot. Pathol. 13: 329–345.CrossRefGoogle Scholar
  2. Barrett, A. J., 1977, Cathepsin D and other carboxyl proteinases, in: Proteinases in Mammalian Cells and Tissues ( A. J. Barrett, ed.), pp. 209–248, North-Holland, Amsterdam.Google Scholar
  3. Christie, G. A., 1966, Implantation in the rat embryo: Glycogen and alkaline phosphatases, J. Reprod. Fertil. 12: 279.CrossRefGoogle Scholar
  4. DeFeo, V. J., 1963a, Temporal aspects of uterine sensitivity in the pseudopregnant or pregnant rat. Endocrinology 72: 305.CrossRefGoogle Scholar
  5. DeFeo, V. J., 1963b, Determination of the sensitive period in the rat by different inducing procedures. Endocrinology 73: 488.CrossRefGoogle Scholar
  6. Denker, H.-W., 1977, Implantation: The role of proteinases and blockage of implantation by proteinase inhibitors, Adv. Anat. Embryol. Cell Biol. 53, part 5.Google Scholar
  7. El-Shershaby, A. M., and Hinchliffe, J. R., 1975, Epithelial autolysis during implantation of the mouse blastocyst: An ultrastructural study, J. Embryol. Exp. Morphol. 33: 1067–1080.Google Scholar
  8. Enders, A. C., and Given, R. L., 1977, The endometrium of delayed and early implantation, in: Biology of the Uterus ( R. M. Wynn, ed.), pp. 203–243, Plenum Press, New York.Google Scholar
  9. Enders, A. C., and Schlafke, S., 1967, A morphological analysis of the early implantation states in the rat, Am. J. Anat. 120: 185–226.CrossRefGoogle Scholar
  10. Fainstat, T., 1963, Extracellular studies of the uterus. I. Disappearance of the discrete collagen bundles in endometrial stroma during various reproductive states in the rat. Am. J. Anat. 112: 337–369.CrossRefGoogle Scholar
  11. Finn, C. A., 1965, Oestrogen and the decidual cell reaction of implantation in mice, J. Endocrinol. 32: 223.CrossRefGoogle Scholar
  12. Finn, C. A., 1977, The implantation reaction, in: Biology of the Uterus ( R. M. Wynn, ed.), pp. 245 - 308, Plenum Press, New York.Google Scholar
  13. Finn, C. A., and Bredl, J. C. S., 1973, Studies on the development of the implantation reaction in the mouse uterus: Influence of actinomycin D, J. Reprod. Fertil. 34: 247.CrossRefGoogle Scholar
  14. Finn, C. A., and Bredl, J. C. S., 1977, Autoradiographic study of the effect of actinomycin D on decidual differentiation of stromal cells in the mouse uterus, J. Reprod. Fertil. 50: 109–111.CrossRefGoogle Scholar
  15. Henzl, M. R., Smith, R. E., Boost, G., and Tyler, E. T., 1972, Lysosomal concept of menstrual bleeding in humans, J. Clin. Endocrinol. 34: 860.CrossRefGoogle Scholar
  16. Hinchliffe, J. R., and El-Shershaby, A. M., 1975, Epithelial cell death in the oil-induced decidual reaction of the pseudopregnant mouse: An ultrastructural study, J. Reprod. Fertil. 45: 463–468.CrossRefGoogle Scholar
  17. Hoversland, R. C., and Weidauf, H. M., 1978, The effect of estrogen and progesterone on the level of amidase activity in fluid flushed from the uteri of ovariectomized mice, Biol. Reprod. 19: 908.CrossRefGoogle Scholar
  18. Jollie, W. P., and Bencosme, S. A., 1965, Electron microscopic observations on primary decidua formation in the rat, Am. J. Anat. 116: 217.CrossRefGoogle Scholar
  19. Lobel, B. L., Tic, L., and Shelesnyak, M. C., 1965, Studies on the mechanism of nidation: Histochemical analysis of decidualization in the rat. Acta Endocrinol. 50: 469.Google Scholar
  20. Lundkvist, J., Ljungkvist, J., and Nilsson, O., 1977, Early effects of oil on rat uterine epithelium sensitized for decidual induction, J. Reprod. Fertil. 51: 507–509.CrossRefGoogle Scholar
  21. Mahaboob Basha, S. M., Horst, M. N., Bazer, F. W., and Roberts, R. M., 1978, Peptidases from pig reproductive tract: Purification and properties of aminopeptidases from uterine secretions, allantoic fluid, and amniotic fluid, Biochem. Biophys. 185: 174.CrossRefGoogle Scholar
  22. Manning, J. P., Steinetz, B. G., Giannina, T., and Meh, A., 1967, Effect of estrogen and progesterone on uterine acid and alkaline phosphatase and ß-glucuronidase activity in mated ovariectomized rats, Proc. Soc. Exp. Biol. Med. 125: 508–512.Google Scholar
  23. Mato, M., Smiley, G. R., and Dixon, A. D., 1972, Epithelial changes in the presumptive regions of fusion during secondary palate formation, J. Dent. Res. 51: 1451–1456.CrossRefGoogle Scholar
  24. Moulton, B. C., 1974, Ovum implantation and uterine lysosomal enzyme activity, Biol. Reprod. 10: 543–548.CrossRefGoogle Scholar
  25. Moulton, B. C., Koenig, B. B., and Borkan, S. C., 1978, Uterine lysosomal enzyme activity during ovum implantation and early decidualization, Biol. Reprod. 19: 167–170.CrossRefGoogle Scholar
  26. Parr, M. B., and Parr, E. L., 1974, Uterine luminal epithelium: Protrusions mediate endocytosis, not apocrine secretion, in the rat, Biol Reprod. 11: 220.CrossRefGoogle Scholar
  27. Parr, M. B., and Parr, E. L., 1978, Uptake and fate of ferritin in the uterine epithelium of the rat during early pregnancy, J. Reprod. Fertil. 52: 183–188.CrossRefGoogle Scholar
  28. Pratt, R. M., and Martin, G. R., 1975, Epithelial cell death and cyclic AMP increase during palatal development, Proc. Natl. Acad. Sci. USA 72: 874–877.CrossRefADSGoogle Scholar
  29. Rankin, J. C., Ledford, B. E., and Baggett, B., 1977, Early involvement of cyclic nucleotides in the artificially stimulated decidual cell reaction in the mouse uterus, Biol. Reprod. 17: 549–554.CrossRefGoogle Scholar
  30. Rosenfeld, M. G., and Joshi, M. S., 1977, A possible role of a specific uterine fluid peptidase in implantation in the rat, J. Reprod. Fértil. 51: 137.CrossRefGoogle Scholar
  31. Saunders, J. W., Jr., 1966, Death in embryonic systems. Science 154: 604–612.CrossRefADSGoogle Scholar
  32. Schlafke, S., and Enders, A. C., 1975, Cellular basis of interaction between trophoblast and uterus at implantation, Biol. Reprod. 12: 41–65.CrossRefGoogle Scholar
  33. Schweichel, J.-U., and Merker, H.-J., 1973, The morphology of various types of cell death in prenatal tissues, Teratology 7: 253–266.CrossRefGoogle Scholar
  34. Smith, M. S. R., and Wilson, I. B., 1971, Histochemical observations on early implantation in the mouse, J. Embryol Exp. Morphol. 25: 165.Google Scholar
  35. Szego, C. M., 1975, The lysosome in nucleocytoplasmic communication, in: Lysosomes in Biology and Pathology ( J. T. Dingle and R. T. Dean, eds.). Vol. 4, pp. 385–477, North-Holland, Amsterdam.Google Scholar
  36. Szego, C. M., Nazareno, M. B., and Porter, D. D., 1977, Estradiol-induced redistribution of lysosomal proteins in rat preputial gland, J. Cell Biol. 73: 354.CrossRefGoogle Scholar
  37. Tachi, S., Tachi, C., and Lindner, H. R., 1970, Ultrastructural features of blastocyst attachment and trophoblastic invasion in the rat, J. Reprod. Fertil. 21: 37–56.CrossRefGoogle Scholar
  38. Woessner, J. F., Jr., 1969, The physiology of the uterus and mammary gland, in Lysosomes in Biology and Pathology (J. T. Dingle and H. B. Fell, eds.). Vol. 1, pp. 299–329, Wiley, New York.Google Scholar
  39. Wood, C., and Psychoyos, A., 1967, Activité de certaines enzymes hydrolytiques dans états de réceptivité utérine chez la ratte, C.R. Acad. Sci. 265: 141–144.Google Scholar
  40. Wood, J. C., 1969, Effect of an intrauterine thread on acid cathepsin activity in the endometrium of the pseudo pregnant rat, Biochem. J. 114: 665–666.Google Scholar
  41. Wood, J. C., 1973, Lysosomes of the uterus, Adv. Reprod. Physiol. 6: 221–230.Google Scholar
  42. Wood, J. C., and Barley, V. L., 1970, Biochemical changes in forming and regressing deciduoma in the rat uterus, J. Reprod. Fertil. 23: 469–475.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Bruce C. Moulton
    • 1
    • 2
  • Sudha Elangovan
    • 1
    • 2
  1. 1.Department of Obstetrics and GynecologyUniversity of Cincinnati College of MedicineCincinnatiUSA
  2. 2.Department of Biological ChemistryUniversity of Cincinnati College of MedicineCincinnatiUSA

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