Polyploidy pp 521-528 | Cite as

Polyploidy, Plants, and Electrophoresis

  • Bruce Carr
  • George Johnson
Part of the Basic Life Sciences book series (BLSC, volume 13)

Abstract

In the last fifteen years the ability to characterize levels of genetic variation in animals using electrophoresis has produced a revolution of knowledge and interest. Similar studies in plants have lagged behind. A review of animal studies (1) lists 129 species (ten systems criterion), while a parallel review of plant studies (2) lists only 61 species, few of them investigated in comparable detail. The reasons for this disparity are partly technical and partly concern the different approach that plant biologists have utilized in their electrophoretic research. This paper attempts to document some of the present technical limitations and conceptual opportunities offered to plant biologists by electrophoresis.

Keywords

Starch Dioxide Phenol DMSO Electrophoresis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Powell, J., 1975, Protein variation in natural populations of animals. Evol. Biol. 8: 79–119.Google Scholar
  2. 2.
    Hamrick, J., 1979, Genetic variation and longevity, pp. 84-113, in Solbrig, O.T., et al. (eds.), “Topics in Plant Population Biology,” Columbia University Press, New York.Google Scholar
  3. 3.
    Kelley, W.A., Adams, R.P., 1977, Preparation of extracts from juniper leaves for electrophoresis. Phytochem. 16: 513–516.CrossRefGoogle Scholar
  4. Anderson, J.W., 1968, Extraction of enzymes and subcellular organelles from plant tissues. Phytochem. 7: 1973–1988CrossRefGoogle Scholar
  5. 5.
    Enama, M., 1977, Molecular weight variation of phosphoenolpyruvate carboxylases from C4 plants. Carnegie Inst. Wash. Yearb. 75: 409–410.Google Scholar
  6. 6.
    Johnson, G., 1976, Hidden alleles at the a-glycerophosphate dehydrogenase locus in Colias butterflies. Genetics 83: 149–167.PubMedGoogle Scholar
  7. 7.
    Singh, R., Lewontin, R., Felton, A., 1976, Genetic heterogeneity within electrophoretic “alleles” of xanthine dehydrogenase in Drosophila pseudoobscura. Genetics 84: 609–629.PubMedGoogle Scholar
  8. 8.
    Coyne, J., 1976, Lack of genie similarity between two sibling species of Drosophila as revealed by varied techniques. Genetics 84: 593–607.PubMedGoogle Scholar
  9. 9.
    Johnson, G., Simonsen, V., Pohlman, C., 1979, The influence of temperature on the electrophoretic behavior of polymorphic enzymes alleles. ( Submitted to Biochem. Genetics )Google Scholar
  10. 10.
    Johnson, G., 1979, Genetically controlled variation in conformation of enz3nnes. Prog. Nucleic Acid Res. J. Molec. Biol. 22: 293–326.CrossRefGoogle Scholar
  11. 11.
    Allard, R.W., Kahler, A.L., Clegg, M.T., 1977, Estimation of mating cycle components of selection in plants. Lecture Notes in Biomath. 19: 1–21.Google Scholar
  12. 12.
    Nei, M., 1972, Genetic distance between population. Amer. Nat. 106: 283–292.CrossRefGoogle Scholar
  13. 13.
    Johnson, G., 1977, Assessing electrophoretic similarity: the problem of hidden heterogeneity. Ann. Rev. Ecol. Syst. 8: 309–328.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Bruce Carr
    • 1
  • George Johnson
    • 1
  1. 1.Department of BiologyWashington UniversitySt. LouisUSA

Personalised recommendations