Summary
Soybean (Glycine max L., Merr.) seeds produce large amounts of protein during the period of rapid seed expansion. Storage proteins comprise 60 to 80% of the accumulated proteins. Messenger RNAs (mRNAs) isolated from developing seeds ranged from approximately 8S to 25S, and in vitro translation reactions containing the RNAs produced two subunits of the 7S storage protein (conglycinin) and polypeptides which may be some or all of the acidic and basic subunits of the 11S protein (glycinin). Electrophoresis of fractionated mRNAs in several gel systems separated several species of mRNA (Mr = 1.1, 0.84, and possibly 0.75 × 106) in fractions of mRNA that code for the in vitro synthesis of subunits of the 7S protein. No other species of RNA was prominent. Complementary DNA produced using the high Mr mRNAs as templates included nearly full-length transcripts.
This research was partially supported by a research grant to RNB from the U.S. Plant, Soil and Nutrition Laboratory, Ithaca, NY. KAB was supported by Fellowship Funds from W.S. and E.M. Meyers.
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References
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© 1980 Plenum Press, New York
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Beachy, R.N., Barton, K.A., Madison, J.T., Thompson, J.F., Jarvis, N. (1980). The mRNAs that Code for Soybean Seed Proteins. In: Leaver, C.J. (eds) Genome Organization and Expression in Plants. NATO Advanced Study Institutes Series, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3051-6_23
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