A New Method for Simplified Prostaglandin Profiling by Selected Ion Monitoring
We have lately been interested in a feasibility study of the experimental requirements that would apply to the simultaneous assay of various classes of prostaglandins, like PGAs, PGBs, PGEs and PGFs, by combined gas chromatography-mass spectrometry (GC-MS). The aim of such a study was the development of a “simple” (in terms of sample handling and clean up procedures) and at the same time practical extraction and derivatization approach suitable for GC-MS applications. In general, many of the reported analytical procedures were deemed rather cumbersome (1), not readily reproducible in our hands or too restrictive in scope, as they are useful only for the determination of a given prostaglandin. In this sense one of the first operational requirements that had to be satisfied due to the analytical constraints imposed by the limited sample preparation procedure was the use of the high selectivity inherent to the specific ion detection techniques. In other words, any possible lack of sufficient sample purification, due to the simplification of the enrichment process as well as to any lack of gas chromatographic resolution prior to the MS analysis, would be compensated by resorting to a very highly selective method of detection.
KeywordsManifold Boronated Helium Prostaglandin PGE2
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