Advertisement

In Vitro Assays for Directing Therapy of Genitourinary Cancers

  • Jonathan Fleischmann
  • Warren D. W. Heston
  • William R. Fair
  • Michael M. Lieber
Part of the Cancer Treatment and Research book series (CTAR, volume 19)

Abstract

There exist only speculative chemotherapy protocols for most genitourinary malignancies. With the exception of non-seminomatous testicular carcinoma, most patients with metastatic cancer will have an uncertain response to chemotherapy. While predictive in vitro chemosensitivity testing for various malignancies has lately been exploited at the commercial level, no one type of test has gained the confidence accorded disc sensitivity testing for infectious diseases.

Keywords

Renal Cell Carcinoma Soft Agar Single Cell Suspension Transitional Cell Carcinoma Clonogenic Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Dexter DL, Calabresi P: Intraneoplastic diversity. Biochem Biophys Acta 695:97–112, 1982.PubMedGoogle Scholar
  2. 2.
    Tsuruo T, Fidler U: Differences in drug sensitivity among tumor cells from parental tumors, selected variants, and spontaneous metastases. Cancer Res 41:3058–3064, 1981.PubMedGoogle Scholar
  3. 3.
    Von Hoff DD, Casper J, Bradley E, Sandbach J, Jones D, Makuch R: Association between human tumor colony-forming assay results and response of an individual patient’s tumor to chemotherapy. Am J Med 70:1027–1032, 1981.CrossRefGoogle Scholar
  4. 4.
    Alberts DS, Salmon SE, Chen HSG, Moon TE, Young L, Surwit EA: Pharmacologic studies of anticancer drugs with the human tumor stem cell assay. Cancer Chem Pharm 6:253–264, 1981.Google Scholar
  5. 5.
    Salmon SE, Hamburger AW, Soehnlen B, Durie BGM, Alberts DS, Moon TE: Quantification of differential sensitivity of human tumor stem cells to anticancer drugs. N Engl J Med 298(24):1321–1327, 1978.PubMedCrossRefGoogle Scholar
  6. 6.
    Wright JC, Cobb JP, Gumport S, Golomb FM, Safadi D: Investigation of the relation between clinical and tissue culture response to chemotherapeutic agents on human cancer. N Engl J Med 257:1207–1211, 1957.PubMedCrossRefGoogle Scholar
  7. 7.
    Wright JC, Cobb JP, Gumport SL, Safadi D, Walker DG, Golomb FM: Further investigation of the relation between the clinical and tissue culture response to chemotherapeutic agents on human cancer. Cancer 15:284–293, 1962.PubMedCrossRefGoogle Scholar
  8. 8.
    Hurley JD, Yount LJ: Selection of anticancer drugs for palliation using tissue culture sensitivity studies. Am J Surg 109:39–42, 1965.PubMedCrossRefGoogle Scholar
  9. 9.
    Dendy PP, Bozman G, Wheeler TK: In vitro screening test for human malignant tumors before chemotherapy. Lancet 2:68–72, 1970.CrossRefGoogle Scholar
  10. 10.
    Lazarus H, Tegeler W, Mazzone HM, Leroy JG, Boone BA, Foley GE: Determination of sensitivity of individual biopsy specimens to potential inhibitory agents: evaluation of some expiant culture methods as assay systems. Can Chem Rep 50(8):543–555, 1966.Google Scholar
  11. 11.
    Wheeler T, Dendy P, Dawson A: Assessment of an in vitro screening test of cytotoxic agents in the treatment of adrenal malignant disease. Oncology 30:362–376, 1974.PubMedCrossRefGoogle Scholar
  12. 12.
    Berry RJ, Laing AH, Wells J: Fresh expiant culture of human tumors in vitro and the assessment of sensitivity to cytotoxic chemotherapy. Br J Cancer 31:218–222, 1975.PubMedCrossRefGoogle Scholar
  13. 13.
    Black MM, Speer FD: Further observations of the effects of cancer chemotherapeutic agents on the in vitro dehydrogenase activity of cancer tissue. J Natl Cancer Inst 14(5):1147–1158, 1954.PubMedGoogle Scholar
  14. 14.
    DiPaolo JA: Analysis of individual chemotherapy assay system. Natl Cancer Inst Monogr 34:240–245, 1971.PubMedGoogle Scholar
  15. 15.
    Kondo T: Prediction of response of tumor and host to cancer chemotherapy. Natl Cancer Inst Monogr 34:251–256, 1971.PubMedGoogle Scholar
  16. 16.
    Roper PR, Drewinko B: Comparison of in vitro methods to determine drug-induced cell lethality. Cancer Res 36:2182.2188, 1976.Google Scholar
  17. 17.
    Shrivastav S, Paulson DF: In vitro chemotherapy testing of transitional cell carcinoma. Invest Urol 17(5):395–400, 1980.PubMedGoogle Scholar
  18. 18.
    Sarosdy MF, Lamm DL, Radwin HM, Von Hoff DD: Clonogenic assay and in vitro chemosensitivity testing of human urologic malignancies. Cancer 50:1332–1338, 1982.PubMedCrossRefGoogle Scholar
  19. 19.
    Durkin WJ, Ghanta VK, Balch CM, Davis DW, Hiramoto RN: A methodological approach to the prediction of anticancer drug effect in humans. Cancer Res 39:402–407, 1979.PubMedGoogle Scholar
  20. 20.
    Weisenthal LM, Dill PL, Kurmick NB, Lippman ME: Comparison of dye exclusion assays with a clonogenic assay in the determination of drug-induced cytotoxicity. Cancer Res 43:258–264, 1983.PubMedGoogle Scholar
  21. 21.
    Buick RN, Stanisic TH, Salmon SE, Trent JM, Krasovich P: Development of an agarmethylcellulose clonogenic assay for cells in transitional cell carcinoma of the human bladder. Cancer Res 39:5051–5056, 1979.PubMedGoogle Scholar
  22. 22.
    Bickis U, Henderson IWD, Quastel JH: Biochemical studies of human tumors (II). Cancer 19:103–113, 1966.PubMedCrossRefGoogle Scholar
  23. 23.
    Tanigawa N, Kern DH, Hikasa Y, Morion DL: Rapid assay for evaluating the chemosensitivity of human tumors in soft agar culture. Cancer Res 42:2159–2164, 1982.PubMedGoogle Scholar
  24. 24.
    Friedman HM, Glaubiger DL: Assessment of in vitro drug sensitivity of human tumor cells using [3H]-thymidine incorporation in a modified human tumor stem cell assay. Cancer Res 42:4683–4689, 1982.PubMedGoogle Scholar
  25. 25.
    Bender RA, Bleyer WA, Drake JC, Ziegler JL: In vitro correlates of clinical responses to methotrexate in acute leukemia and Burkitt’s lymphoma. Br J Cancer 34:484–492, 1976.PubMedCrossRefGoogle Scholar
  26. 26.
    Von Hoff DD, Weisenthal L: In vitro methods to predict for patient response to chemotherapy. Adv Pharm Chem 17:133–156, 1980.Google Scholar
  27. 27.
    Group for Sensivity Testing of Tumors (KSST): In vitro short-term test to determine the resistance of human tumors to chemotherapy. Cancer 48:2127–2135, 1981.CrossRefGoogle Scholar
  28. 28.
    Hart JS, George SL, Frei E, Bodney GP, Nickerson RC, Freilich EJ: Prognostic significance of Pretreatment proliferative activity in adult leukemia. Cancer 39:1603–1617, 1977.PubMedCrossRefGoogle Scholar
  29. 29.
    Tchao R, Easty GC, Ambrose EJ, Raven RW, Bloom HJG: Effect of chemotherapeutic agents and hormones on organ cultures of human tumors. Eur J Cancer 4:39–44, 1968.PubMedGoogle Scholar
  30. 30.
    Shrivastav S, Bonar RA, Stone KR, Paulson DF: An in vitro assay procedure to test chemotherapeutic drugs on cells from human solid tumors. Cancer Res 40:4438–4442, 1980.PubMedGoogle Scholar
  31. 31.
    Tannock I: Cell kinetics and chemotherapy: a critical review. Cancer Treat Rep 62(8): 1117–1133, 1978.PubMedGoogle Scholar
  32. 32.
    Livingston RB, Titus GA, Heilbrun LK: In vitro effects on DNA synthesis as a predictor of biological effect from chemotherapy. Cancer Res 40:2209–2212, 1980.PubMedGoogle Scholar
  33. 33.
    De Sombre ER, Carbone PP, Jensen EU, McGuire WL, Wells SA, Wittliff JL, Lipsett MB: Steroid receptors in breast cancer. N Engl J Med 301:1011–1012, 1979.CrossRefGoogle Scholar
  34. 34.
    Bloom HJG: Hormone-induced and spontaneous regression of metastatic renal cancer. Cancer 32:1066–1071, 1973.PubMedCrossRefGoogle Scholar
  35. 35.
    Pearson J, Friedman MA, Hoffman PG: Hormone receptors in renal cell carcinoma. Cancer Chem Pharm 6:151–154, 1981.CrossRefGoogle Scholar
  36. 36.
    Ekman P, Snochowski M, Zetterberg A, Hogberg B, Gustafsson JA: Steroid receptor content in human prostatic carcinoma and response to endocrine therapy. Cancer 44:1173–1181, 1979.PubMedCrossRefGoogle Scholar
  37. 37.
    Trachtenberg J, Walsh PC: Correlation of prostatic nuclear androgen receptor content with duration of response and survival following hormonal therapy in advanced prostatic cancer. J Urol 127:466–471, 1982.PubMedGoogle Scholar
  38. 38.
    Alley MC, Uhl CB, Lieber MM: Improved detection of drug cytotoxycity in the soft agar colony formation assay through use of a metabolizable tetrazolium salt. Life Sci 31:3071–3078, 1983.CrossRefGoogle Scholar
  39. 39.
    Editorial: Clonogenic assays for the chemotherapeutic sensitivity of human tumors. Lancet 3:780–781, 1982.Google Scholar
  40. 40.
    Selby P, Buick RN, Tannock I: A critical appraisal of the ‘human tumor stem cell assay’. N Engl J Med 308:129–134, 1983.PubMedCrossRefGoogle Scholar
  41. 41.
    Dow LW, Bhakta M, Wilimas J: Clonogenic assay for Wilm’s tumor: improved technique for obtaining single cell suspensions and evidence for tumor cell specificity. Cancer Res 42:5262–5264, 1982.PubMedGoogle Scholar
  42. 42.
    Slocum HK, Pavelic ZP, Kanter PM, Nowak NJ, Rustum YM: The soft agar clonogenicity and characterizations of cells obtained from human solid tumors by mechanical and enzymatic means. Cancer Chem Pharm 6:219–225, 1981.CrossRefGoogle Scholar
  43. 43.
    MacKintosh FR, Evans TL, Sikic BI: Methodologic problems in clonogenic assays of spontaneous human tumors. Cancer Chem Pharm 6:205–210, 1981.CrossRefGoogle Scholar
  44. 44.
    Hamburger AW, White CP, Tencer K: Effect of enzymatic disaggregation on proliferation of human tumors in soft agar. AACR Abstracts 73:182, 1982.Google Scholar
  45. 45.
    Alberts DS, Salmon SE, Chen HSG, Moon TE, Young L, Surwit EA: Pharmacologic studies of anticancer drugs with the human tumor stem cell assay. Cancer Chem Pharm 6:253–264, 1981.Google Scholar
  46. 46.
    Ludwig R, Alberts DS, Miller TP, Salmon SE, Wood DA: The schedule dependency (SD) of anticancer drugs in the human tumor stem cell assay (HTSCA). AACR Abstracts 73:718, 1982.Google Scholar
  47. 47.
    Lieber MM, Kovach JS, Soft agar clonogic assay for primary renal carcinoma: in vitro chemotherapy drug sensitivity testing. Invest Urol 19:111–114, 1981.PubMedGoogle Scholar
  48. 48.
    Stanisic TH, Buick RN: An in vitro clonal assay for bladder cancer: clinical correlation with the status of the urotheliam in 33 patients. J Urol 124:30–33, 1980.Google Scholar
  49. 49.
    Durie BGM, Persky B, Soehnlen BJ, Grogan TM, Salmon SE: Amyloid production in human myeloma stem-cell culture, with morphologic evidence of amyloid secretion by associated macrophages. N Engl J Med 307:1689–1692, 1982.PubMedCrossRefGoogle Scholar
  50. 50.
    Rosenblum ML, Dougherty DV, Reese C, Wilson CB: Potentials and possible pitfalls of human stem cell analysis. Cancer Chem Pharm 6:227–235, 1981.CrossRefGoogle Scholar
  51. 51.
    Darznkiewicz Z, Traganos F, Staianocoico L, Kapuscinski J, Melamud MR: Interactions of rhodamine 123 with living cells studied by flow cytometry. Cancer Res 42:799–806, 1982.Google Scholar
  52. 52.
    Stanisic TH, Owens R, Graham AR: Use of clonal assay in determination of urothelial drug sensitivity in carcinoma in situ of the bladder: clinical correlations in five patients. J Urol (in press).Google Scholar
  53. 53.
    Sherwin SA, Twardzik DR, Bohn WH, Cockley KD, Todaro GJ: High-molecular-weight transforming growth factor activity in the urine of patients with disseminated cancer. Cancer Res 43:403–407, 1983.PubMedGoogle Scholar

References

  1. 1.
    Salmon SE, Hamburger AW, Soehnlen B, Durie BGM, Alberts DS, Moon TE: Quantification of differential sensitivity of human tumor stem cells to anticancer drugs. N Engl J Med 298(24):1321–1327, 1978.PubMedCrossRefGoogle Scholar
  2. 2.
    Agrez MV, Kovach JS, Lieber MM: Cell aggregates in the soft agar ‘human tumour stem-cell assay’. Br J Cancer 46:880–887, 1982.PubMedCrossRefGoogle Scholar
  3. 3.
    Alley MC, Uhl CB, Lieber MM: Improved detection of drug cytotoxicity in the soft agar colony formation assay through use of a metabolizable tetrazolium salt. Life Sci 31:3071–3078, 1982.PubMedCrossRefGoogle Scholar
  4. 4.
    Lieber MM: Soft agar colony formation assay for in vitro chemotherapy sensitivity testing of human renal cell carcinomas: Mayo Clinic experience. J Urol (submitted).Google Scholar
  5. 5.
    Editorial: Clonogenic assays for the chemotherapeutic sensitivity of human tumors. Lancet 3:780–781, 1982.Google Scholar

Copyright information

© Martinus Nijhoff Publishers, Boston 1984

Authors and Affiliations

  • Jonathan Fleischmann
  • Warren D. W. Heston
  • William R. Fair
  • Michael M. Lieber

There are no affiliations available

Personalised recommendations