Electron microscopy of proteoglycans

  • C. Johan
  • O. Thyberg
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 3)

Abstract

In addition to the fibrillar proteins collagen and elastin, proteoglycans constitute a major macro-molecular component in the extracellular matrix of connective tissues. They have also been identified as components of basement membranes and cell surface coats. Chemically, the proteoglycans consist of a protein core to which glycosaminoglycan chains are covalently attached. The latter are composed of alternating uronic acid and hexosamine residues and are all polyanions with acidic sulfate and/or carboxyl groups (1). A particularly high content of proteoglycans is found in cartilage and much of the information that is available today derives from studies on this tissue. An average cartilage proteoglycan has a molecular weight of about 2.5 af09106 and is composed of a protein core to which about 100 chon-droitin sulfate and 50–60 keratan sulfate chains are bound. Within the cartilaginous matrix, most of these monomers occur in large aggregates, formed by noncovalent interaction with hyaluronic acid and link proteins (2–4). The structure of proteoglycans in other connective tissues, in basement membranes, and in cell surface coats is less well known. The type, number, and size of glycosaminoglycan chains per molecule have been found to vary considerably, but the supramolecular organization (e.g. aggregate formation) is still poorly defined.

Keywords

Permeability Polysaccharide Acidic Sulfate Trypsin Macromolecule 

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© Martinus Nijhoff Publishers, Boston, The Hague, Dordrecht, Lancaster 1984

Authors and Affiliations

  • C. Johan
  • O. Thyberg

There are no affiliations available

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