Ultrastructural Analysis of B- and T-Lymphocyte Subsets with the Immunogold Technique

Its Relevance to the Study of Chronic Lymphoid Leukemias
  • Estela Matutes
  • Daniele Robinson
  • Daniel Catovsky
Part of the Developments in Oncology Series book series (DION, volume 14)

Abstract

Advances in morphological techniques, such as the application of cytochemistry at electron microscope (EM) level and of immunological methods, particularly the development of monoclonal antibodies against antigens of hemopoietic cells, have proved to be of value in the characterization of normal and leukemic cells [1–3]. Several tracers such as immunoperoxidase, ferritin or colloidal gold particles [4] have been used at EM level to localize antigenic sites in different tissues. The immunoperoxidase method has been widely used in hematological material for the study of D-cell neoplasias [3]. Since colloidal gold particles were proposed as a tracer for transmission electron microscopy (TEM) [6] and later for scanning electron microscopy (SEM) [7] studies with this technique in conjunction with monoclonal antibodies have been confined to light microscopy (LM) observations of normal lymphocytes [7, 8].

Keywords

Lymphoma Leukemia Citrate Gall Ferritin 

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Copyright information

© Martinus Nijhoff Publishing, Boston/The Hague/Dordrecht/Lancaster 1984

Authors and Affiliations

  • Estela Matutes
  • Daniele Robinson
  • Daniel Catovsky

There are no affiliations available

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