Neurotransmitter Release Stimulated in a Clonal Nerve Cell Line by Low Intensity Pulse Magnetic Fields

  • G. Rein
  • R. Dixey


Because of our growing interest in the biological activity of electromagnetic fields (EMF), we have set out to establish a model system for studying their mechanism of action at the molecular level. Although in vitro preparations of bone (1,2) and nerve cells (3–6) have been used, the populations are heterogeneous and direct effects on specific membrane events are difficult to monitor. Isolation of specific cell types has been used for red blood cells (7). Separation of neuronal cells, however, requires elaborate time-consuming techniques which are likely to affect the integrity of the membranes i n the final preparation. Single cell clonal lines of neural crest tumors in culture are likely to be useful, since they express many properties associated with normal mature adrenal chromaffin cells (8–13). Neuroblastoma cells are an example of one such line which is being used for EMF studies (14). Recently, Greene and Tischler developed a more differentiated clonal line, PC12, derived from a rat phaeochromocytoma (12) which also exhibits properties of sympathetic neurones including calcium dependent release of neurotransmitters (8,9,11).


PC12 Cell Neurotransmitter Release Noradrenaline Release Pulse Magnetic Field Washout Curve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    R.A. Luben, C.D. Cain, M.C.-Y. Chen, D.M. Rosen and W.R. Adey, Proc. Natl. Acad. Sci. , 79: 4180–4184 (1982).ADSCrossRefGoogle Scholar
  2. 2.
    C. W. Archer and N. A. Ratcliffe, J. Exptl. Zoology 225: 243–256 (1983).CrossRefGoogle Scholar
  3. B.F. Sisken and J.F. Lafferty, J.F. Bioelect. Bioenergetics 5: 459–464 (1979).CrossRefGoogle Scholar
  4. 4.
    S.M. Bawin and W.R. Adey, Proc. Natl. Acad. Sci., USA 73: 1999–2003 (1976).ADSCrossRefGoogle Scholar
  5. W.R. Adey, Physiol. Rev. 61: 435–514, (1981).Google Scholar
  6. 6.
    C.F. Blackman S. A. Elder, C.M. Weil, S.G. Benane, D.C. Eichinger and D.E. House, Radio Science 14: 93–98 (1979).ADSCrossRefGoogle Scholar
  7. 7.
    A. Chiabrera, M. Hinsenkamp A.A. Pilla, J. Ryaby, D. Ponta, A. Belmont, F. Beltrame, M. Grattarola and C. Nicolini, J. Histochem. and Cytochem. 27: 375–381 (1979).Google Scholar
  8. 8.
    L.A. Greene and G. Rein, Brain Res. 129: 247–263 (1977).CrossRefGoogle Scholar
  9. 9.
    L.A. Greene and G. Rein, Brain Res. 138: 521–528 (1977).CrossRefGoogle Scholar
  10. 10.
    L.A. Greene and G. Rein, Nature 268: 349–351 (1977).ADSCrossRefGoogle Scholar
  11. 11.
    A.K. Richie, J. Physiol. (London) 286: 541–561 (1979).Google Scholar
  12. 12.
    L.A. Greene and A.S. Tischler, Proc. Natl. Acad. Sci. USA 73: 2424–2428 (1976).ADSCrossRefGoogle Scholar
  13. 13.
    M.A. Dichter, A.S. Tischler and L.A. Greene, Nature 268: 501–504 (1977).ADSCrossRefGoogle Scholar
  14. 14.
    S.K. Dutta, B. Ghosh, A. Subramonian and R. Parshad, Bioelectromagnetics (in press).Google Scholar
  15. 15.
    B. Kaler, P.O. Byrne, D.D. Milner, Proc. H.P.A. Sheffield England (October 24, 1980 ).Google Scholar
  16. 16.
    M.J. Lunt and B.W. Watson, Clin. Phys. Physiol. Measurement3: 221–225 (1982).ADSCrossRefGoogle Scholar
  17. 17.
    M.J. Lunt, Med. Biol. Eng. Comput. 24: 501–511 (1982).CrossRefGoogle Scholar
  18. 18.
    P. Armitage, Stat Methods in Medical Res., Blackwell Scientific Publication s, Oxford (1971).Google Scholar
  19. 19.
    T.P. Williams and R. McGee, J. Biol. Chem. 257: 3491–3500 (1982).Google Scholar
  20. 20.
    D.M. Paton, in: “Mechanisms of Neuronal Transport,” D.M. Paton (ed.), Raven Press, New York (1976).Google Scholar
  21. 21.
    R. Dixey (unpublished observations).Google Scholar
  22. 22.
    R. Dixey and G. Korenman (unpublished observations).Google Scholar
  23. 23.
    S.M. Bawin, A. Sheppard and W.R. Adey, Bioelectrochemistry and Bioenergetics 3: 67–76 (1978).CrossRefGoogle Scholar
  24. 24.
    R. Dixey and G. Rein, Nature 296: 253–256 (1982).ADSCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • G. Rein
    • 1
  • R. Dixey
    • 1
  1. 1.Department of Medical ElectronicsSt. Bartholomew’s HospitalWest Smithfield LondonEngland

Personalised recommendations