Abstract
In a number of clinical studies (1–3) the usefulness of induced electromagnetic fields in stimulating healing of chronic fracture non-unions has been demonstrated. These fields stimulate a number of activities including the synthesis of new matrix by osteoblasts (4), transcription of genetic information by bone cells (5) and other processes associated with fracture healing. In addition, DC voltage applied to bones by invasive techniques has been reported to stimulate both localized osteolysis (in areas near the anode), and localized osteogenesis (in areas near the cathode), presumably by alteration of the activity of endogenous bone forming and/or bone resorbing cells (6). Externally induced electromagnetic fields used for treatment of fractures produce tissue current densities of less than 1 microampere/cm2 and associated electric gradients of about 1 mV/cm (2). As yet there is limited understanding of the possible mechanisms by which fields of this low magnitude (relative to tissue transmembrane potentials and cellular impedances) could bring about such significant changes in the activity of bone cells in situ.
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References
Bassett, C.A.L., Pilla, A.A. and Pawluk, R.J. A non-operative salvage of surgically-resistant pseudarthroses and non-unions by pulsing electromagnetic fields. Clin. Orthop. Rel. Res. 124: 128–142 (1977)
Brighton, C.T., Black, J., Friedenberg, Z.B., Esterhai, J.L., Day, L.J. and Connoly, J.F. A multicenter study of the treatment of non-union with constant direct current. J. Bone and Joint Surg. . 63-A: 2–12 (1981).
Becker, R.O. Electrical osteogenesis-pro and con. Calc. Tiss. Res. 26: 93–97 (1978).
Luben, R.A., Cain, C.D., Chen, M.C.Y., Rosen, D.M., and Adey, W.R. Effects of electromagnetic stimuli on bone and bone cells in vitro: Inhibition of responses to parathyroid hormone by low-energy low-frequency fields. Proc. Nat. Acad. Sci. (USA). 79: 4180–4184 (1982).
Bassett, C.A.L. Pulsing electromagnetic fields: A new method to modify cell behavior in calcified and noncalcified tissues. Calc. Tiss. Internat. 34: 1–8 (1982).
Bassett, C.A.L. The biological significance of piezoelectricity. Calc. Tiss. Res. 1: 251–272 (1968).
Luben, R.A., Chen, M.C.Y., Rosen, D.M. and Mohler, M.A. Effects of osteoclast activating factor from human lymphocytes on cyclic AMP concentrations in isolated mouse bone and bone cells. Calc. Tiss. International. 28: 23–32 (1979).
Rosen, D.M., and Luben, R.A. Multiple hormonal mechanisms for the control of collagen synthesis in an osteoblast-like cell line, MMB-1. Endocrinology. 112: 992–999 (1983).
Walters, M.R., Rosen, D.M., Norman, A.W., and Luben, R.A. 1,25-Dihydroxyvitamin D receptors in an established bone cell line: Correlation with biochemical responses. J. Biol. Chem. 257: 7481–7484 (1982).
Pilla, A.A. in “Bioelectrochemistry,” ed. Keyzer, H. and Gutman, F. ( Plenum Publishing Corp., New York ), pp. 353–396 (1980).
Rizzoli, R.E., Somerman, M., Murray, T.M., and Aurbach, G. D. Binding of radioiodinated parathyroid hormone to cloned bone cells. Endocrinology. 113:1832–1838 (19083).
Ross, E.M. and Gilman, A.G. Reconstitution of catecholamine-sensitive adenylate cyclase activity: Interaction of solubilized components with receptor-replete membranes. Proc. Nat. Acad. Sci. (USA). 74: 3715–3719 (1977).
Peck, W.A. Cyclic AMP as a second messenger in the skeletal actions of parathyroid hormone: A decade-old hypothesis Calc. Tiss. International. 29: 1–4 (1979).
Kirchick, H.J., and Birnbaumer, L. Effects of estradiol treatment on rabbit luteal adenylyl cyclase: Loss of luteinizing hormone receptors and attenuation of the regulatory N component activity. Endocrinology. 113: 1629–1637 (1983).
Adey, W.R. Tissue interactions with nonionizing electromagnetic fields. Physiol. Revs. 61: 435–514 (1981).
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© 1984 Plenum Press, New York
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Luben, R.A., Cain, C.D. (1984). Use of Bone Cell Hormone Response Systems to Investigate Bioelectromagnetic Effects on Membranes in Vitro . In: Adey, W.R., Lawrence, A.F. (eds) Nonlinear Electrodynamics in Biological Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2789-9_2
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DOI: https://doi.org/10.1007/978-1-4613-2789-9_2
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