Abstract
To determine its chemical composition as well as the mechanism of function. the chloroplast must be prepared in a relatively pure state. An important consideration during purification is the osmolarity of the surrounding medium. In solutions with osmolarities less than that inside the organelle, bulk water is drawn in until the chloroplast swells to the limits of elasticity of its envelope, beyond which it bursts. This condition permits escape of nearly all water-soluble components contained in the stroma of the chloroplast. Such drastic consequences can be avoided, however, by adding to the medium a solute to which the chloroplast envelope is relatively impermeable, at a concentration sufficient to balance the internal osmotic pressure. The two most widely used substances for this purpose are sucrose and sorbitol (Fig. 3.1).
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© 1984 Plenum Press, New York
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Hoober, J.K. (1984). Characteristic Components of Chloroplast Membranes. In: Chloroplasts. Cellular Organelles. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2767-7_4
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DOI: https://doi.org/10.1007/978-1-4613-2767-7_4
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