Influence of Demographic Factors on Scheduled DNA Synthesis as Measured in Human Peripheral Leukocytes and Fibroblasts
In a population study of leukocytes from 140 human subjects, far UV-induced unscheduled DNA synthesis (UDS) variation correlated with at least three demographic factors — smoking tobacco, season of sample obtainment and “street opiate” addiction. The ability to correlate UDS variability with these factors demonstrates the importance of environmental influences in control of DNA repair processes and the need to include the effect of demographics in genetic risk assessment models. In addition, mutagen-induced UDS patterns for mononuclear leukocytes from a single subject did not match the UDS pattern for fibroblasts grown from a forearm skin biopsy from the same individual which suggests that UDS may be tissue specific.
KeywordsRepair Capacity Risk Assessment Model Mononuclear Leukocyte Heroin Addiction Opiate Addiction
Unable to display preview. Download preview PDF.
- 2.B. Lambert, U. Ringborg, and L. Skoog, Age-related decrease in UV light-induced DNA repair synthesis in human peripheral leukocytes, Cancer Res., 39:2792–2795 (1979).Google Scholar
- 4.M. Frey-Wettstein, R. Longmire, and C. G. Craddock, Deoxyribonucleic acid (DNA) repair replication of ultraviolet (UV) irradiated normal and leukemic leukocytes, J. Lab. Clin. Med., 74:109–118 (1972).Google Scholar
- 6.F.-H. Yew and R. T. Johnson, Ultraviolet-induced DNA excision repair in human B and T lymphocytes. III. Repair in lymphocytes from chronic lymphocytic leukaemia, J. Cell Sci., 39:329–337 (1979).Google Scholar
- 11.K. Erixon and G. Ahnstrom, Single strand breaks in DNA during repair of UV-induced damage in normal human and xeroderma pigmentosum cells as determined by alkaline DNA unwinding and hydroxylapatite chromatography. Effects of hydroxyurea, 5-fluoro-deoxyuridine and 1-3-D-arabinofuranosylcytosine on the kinetics of repair, Mutat. Res., 59:257–271 (1979).CrossRefGoogle Scholar
- 13.N. Prashad and R. Cutler, Percent satellite DNA as a function of tissue and age of mice, Biochem. Biophys. Act., 418:1–23 (1976).Google Scholar
- 14.K. W. Giles and A. Myers, An improved diphenylamine method for the estimation of DNA, Nature, 4979:93 (1965).Google Scholar
- 16.B. Paigen, E. Ward, A. Reilly, L. Horton, H. L. Gurtoo, J. Minowada, K. Steenland, M. B. Havens, and P. Sartori, Seasonal variation of aryl hydrocarbon hydroxylase activity in human lymphocytes, Cancer Res., 41:2757–2761 (1981).Google Scholar
- 17.E. W. Van Carter, E. Virasoro, R. Le Clercq, and G. Copinschi, Seasonal, circadian, and episodic variations of human immunereactive β-MSH, ACTH, and Cortisol, Int. J. Pept. Protein Res., 17:3–13 (1981).Google Scholar
- 20.R. J. McDonough, J. J. Madden, A. Falek, D. A. Shafer, M. Pline, D. Gordon, P. Bokos, J. C. Kuehnle, and J. Mendelson, Alteration of T and null lymphocyte frequencies in the peripheral blood of human opiate addicts:in vivo evidence for opiate receptor sites on T lymphocytes, J. Immunol., 125:2539–2543 (1980).Google Scholar
- 21.A. Melander, E. Nordenskjold, B. Lundh, J. Thorell, Influence of smoking on thyroid activity, Acata Med. Scan., 209 (1–2):41–43 (1981).Google Scholar