Abstract
The study of chromosomal aberrations in lymphocytes is a sensitive but time consuming method for revealing mutagen effects in humans. An interesting alternative and/or complement could be to use micronuclei as an indicator of chromosomal damage. Micronuclei are formed during cell division from acentric fragments or lagging chromosomes and thus reflect both damage of the chromosomes and the spindle apparatus. They are easily detected in interphase cells as free intracytoplasmatic bodies of nuclear origin.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
P. J. Countryman and J. A. Heddle, The production of micro- nuclei from chromosome aberrations in irradiated cultures of human lymphocytes, Mutation Research, 41:321–332 (1976).
J. A. Heddle, C. V. Lue, E. F. Saunders, and R. D. Benz, Sensitivity to five mutagens in Fanconi’s anemia as measured by the micronucleus method, Cancer Research, 38:2983–2988 (1978).
A. Norman, F. H. Adams, and R. F. Riley, Cytogenetic effects of contrast media and triiodobenzoic acid derivatives in human lymphocytes, Radiology, 129:199–203 (1978).
K. Linnainmaa, T. Meretoja, M. Sorsa, and H. Vainio, Cytogenetic effects of styrene and styrene oxide on human lymphocytes and Allium cepa, Scand. J. Work Environ, and Health, 4: 156–162 (1978).
B. Htfgstedt, B. Gullberg, K. Hedner, A.-M. Kolnig, F. Mitelman, and S. Skerfving, Chromosome aberrations and micronuclei in bone marrow cells and peripheral blood lymphocytes in humans exposed to ethylene oxide, Hereditas, 98:105–113 (1983).
K. Sugiura, T. Kimura, and M. Goto, Mutagenicities of styrene oxide derivatives on Salmonella typhimurium (TA 100), Mutation Research, 58:159–165 (1978).
M. Donner, M. Sorsa, and H. Vainio, Recessive lethals induced by styrene and styrene oxide in Dorosophila melanogaster, Muta- tation Research, 67:373–376 (1979).
L. Fabry, A. Leonard, and M. Roberfroid, Mutagenicity tests with styrene oxide in mammals, Mutation Research, 51:377–381 (1978).
H. Norypa, Thein vitroinduction of sister chromatid exchanges and chromosome aberrations in human lymphocytes by styrene derivatives, Carcinogenesis, 2:237–242 (1981).
T. Meretoja, H. Vainio, M. Sorsa, and H. H£rk8nen, Occupational styrene exposure and chromosomal aberrations, Mutation Research, 56:193–197 (1977).
B. HSgstedt, K. Hedner, E. Mark-Vendel, F. Mitelman, A. Schutz, and S. Skerfving, Increased frequency of chromosome aberrations in workers exposed to styrene, Scand. J. Work Environ, and Health, 5:333–335 (1979).
H. C. Andersson, E. A. Tranberg, A. H. Uggia, and G. Zetterberg, Chromosomal aberrations and sister chromatid exchanges in lymphocytes of men occupationally exposed to styrene in a plastic boat factory, Mutation Research, 73:387–401 (1980).
M. G. Ott, R. C. Kolesar, H. C. Scharnweber, E. J. Schneider, and J. R. Venable, A mortality survey of employees engaged in the development or manufacture of styrene-based products, J. Occupational Med., 22:445–460 (1980).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Högstedt, B., Mitelman, F. (1984). Micronuclei in Cultured Lymphocytes as an Indicator of Genotoxic Exposure. In: de Serres, F.J., Pero, R.W. (eds) Individual Susceptibility to Genotoxic Agents in the Human Population. Environmental Science Research, vol 30. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2765-3_13
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2765-3_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9709-3
Online ISBN: 978-1-4613-2765-3
eBook Packages: Springer Book Archive