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Electron Microscopic Mapping and Ultrastructure of Drosophila Polytene Chromosomes

  • Veikko Sorsa

Abstract

The number of molecular studies involving cloned segments of DNA has been growing rapidly. This development holds a promise of rapid progress in the study of individual genes of Drosophila melanogaster. The exact sequences of DNA of several genes evidently will be available in the near future (cf. Spradling and Rubin, 1981). Consequently, the cytogenetic localization of cloned sequences on the chromosomes is also going to meet new challenges. More exact cytological methods have to be developed to demonstrate the normal banding pattern of polytene chromosomes and to recognize minute rearrangements in it. The quality and usability of all new methods should be tested against the revised light microscopic reference maps of Bridges (cf. Lindsley and Grell, 1968). An urgent task, however, is the reliable verification and possible revision of light microscopic maps by means of high-resolution electron microscopy. EM division maps for the salivary gland chromosome 2L of D. melanogaster are included in this review.

Keywords

Salivary Gland Banding Pattern Polytene Chromosome Chromatin Fiber Double Band 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • Veikko Sorsa
    • 1
  1. 1.Department of GeneticsUniversity of HelsinkiHelsinki 10Finland

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