Abstract
The application of Differential Scanning Calorimetry (DSC) to biological problems is relatively recent. DSC is proving to be a powerful approach not only for investigating structural problems, but for studies on physiological processes as well. DSC has had its most notable biological successes in studies of lipid containing systems (1,2). The intent of this chapter is to briefly describe some calorimetric approaches our laboratory has used to obtain information on these systems.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
D. L. Melchior and J. M. Steim, Lipid-associated thermal events in biomembranes, Prog. Surf. Membr. Sci. 13: 211–296 (1979).
R. N. McElhaney, The use of differential scanning calorimetry and differential thermal analysis in studies of model and biological membranes, Chem. Phys. Lipids 30:229–259 (1982).
D. M. Small, The physical state of lipids of biological importance: Cholesterol ester, cholesterol, triglycerids, Adv. Exp. Med. Biol. 7:55–83 (1970).
D. L. Melchior, E. P. Bruggemannand J. M. Steim, The physical state of quick-frozen membranes and lipids, Biochim. Biophys. Acta 690:81–88 (1982).
A. Carruthers and D. L. Melchior, Cytosolic modulation of human erythrocyte hexose transfer, Biochim. Biophys. Acta 728:254–266 (1983).
L. Thilo, H. Trauble, and P. Overath, Mechanistic interpretation of the influence of lipid phase transitions on transport functions, Biochemistry 16: 1283–1290 (1977).
D. L. Melchior and J. M. Steim, Thermotropic transitions in biomembranes, Annu. Rev. Biophys. Bioeng. 5:205–238 (1976).
D. L. Melchior, Lipid phase transitions and regulation of membrane fluidity in prokaryotes, in: “Current Topics in Membranes and Transport, Vol. 17” S. Razin and S. Rottem, Eds. Academic Press, New York, (1982).
D. L. Melchior and J. M. Steim, Control of fatty acid composition Acholeplasma laidlawii membranes, Biochim. Biophys. Acta 466:148–159 (1977)
R. A. Demel and B. de Kruyff, The function of sterols in membranes, Biochim. Biophys. Acta 457: 109–132 (1976).
R. Bittman and L. Blau, The phospholipid-cholesterol interaction. Kinetics of water permeability in liposomes, Biochemistry 11: 4831–4839 (1972).
D. L. Melchior and M. P. Czech, Sensitivity of the adipocyte transport system to membrane fluidity in reconstituted vesicles, J. Biol. Chem. 254:8744–8747 (1979).
D. L. Melchior, F. J. Scavitto, and J. M. Steim, Dilatometry of dipalmitoyllecithin-cholesterol bilayers, Biochemistry 19: 4828–4834.
R. A. Demel, J. W. C. M. Jansen, P. W. M. van Dijck, and L. L. M. van Deenen, The preferential interaction of cholesterol with different classes of phospholipids, Biochim. Biophys. Acta 465:1–10 (1977).
E. P. Bruggemann and D. L. Melchior, Alterations in the organization of phosphotidylcholine/cholesterol bilayers by THC, J. Biol. Chem.: in press.
M. F. Either, D. E. Wolf and D. L. Melchior, A Calorimetric investigation of the phase partitioning of the fluorescent carboxyanine probes in phosphatidylcholine bilayers, Biochemistry 22: 1172–1178 (1983).
D. L. Melchior and S. Rottem, Organization of cholesterol esters in the membranes of Mycoplasma capricolum, Eur. J. Biochem. 117:147–153 (1981).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Melchior, D.L. (1984). Some Uses of Differential Scanning Calorimetry in Biomembrane Research. In: Johnson, J.F., Gill, P.S. (eds) Analytical Calorimetry. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2699-1_26
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2699-1_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9677-5
Online ISBN: 978-1-4613-2699-1
eBook Packages: Springer Book Archive