Abstract
Human plasma is an extraordinarily complex aqueous solution of proteins, hormones, lipoids, carbohydrates etc., from which certain proteins are of interest in therapy. At the moment a small but increasing number of the hundreds of plasma proteins are used in clinical medicine and have to be prepared by fractionation. The methods applied in fractionation are more or less the same as for purification of proteins, but the aim is quite different; fractionation is meant to make the most out of plasma in terms of the clinical efficacy of the wanted protein(s). The safety of the preparations must be guaranteed by avoiding possible changes in the native structure of the protein(s) or contamination that causes side effects. The economics of fractionation also have to be considered. To reach this aim, good recovery and efficacy of the protein (s) are necessary and are mainly dependent on controlled methods and technology. The safety is dependent mainly on a correct interpretation of “Good Manufacturing Practices”, which primarily is to be found in well instructed and well educated personnel, adequate housing, sanitation and equipment. Continuous attention is needed to control the costs.
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© 1985 Martinus Nijhoff Publishing, Boston
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Brummelhuis, H.G.J. (1985). Ethanol Fractionation of Human Plasma: An Overview. In: Smit Sibinga, C.T., Das, P.C., Seidl, S. (eds) Plasma Fractionation and Blood Transfusion. Developments in Hematology and Immunology, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2631-1_7
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DOI: https://doi.org/10.1007/978-1-4613-2631-1_7
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