Monocyte Contrasuppression of EBV-Immune Regulatory T Cells

  • G. Tosato
  • S. E. Pike
  • R. M. Blaese
Part of the Developments in Medical Virology book series (DIMV, volume 1)


EBV-immune normal individuals have circulating regulatory T cells that inhibit the activation of autologous B cells by EBV. When T cells were fractionated in subsets enriched for either T8 or T4 expressing lymphocytes and subsequently cultured with autologous EBV-infected cells, most of the inhibitory activity derived from T8-enriched populations. Monocyte depletion of EBV immune autologous B and T cells was associated with an enhanced degree of T-cell inhibition. Conversely, monocyte reconstitution of monocyte-depleted cultures markedly reduced EBV-immune T-cell suppression. Thus, in an EBV-specific system of suppression, monocytes act as regulatory cells with “contrasuppressor” function.


Late Suppression Major Histocompatability Complex Iron Carbonyl Immunoregulatory Mechanism Suppression Immunoglobulin 
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  1. Bianco, C., Plasma membrane receptors for complement. In Biological Amplification Systems in Immunology. N.K. Day and R.A. Good, eds. Rockefeller University, New York pp. 69–84 (1977).Google Scholar
  2. Bird, A.G., and Britton, S., A new approach to the study of human B lymphocyte function using an indirect plaque assay and a direct B cell activator. Immunol. Rev. 45, 41–66 (1979).PubMedCrossRefGoogle Scholar
  3. Diehl, V., Henle, G., Henle, W., and Kohn, G., Demonstration of a herpes group virus in cultures of peripheral leukocytes from patients with infectious mononucleosis. J. Virol. 2, 663–669 (1968).PubMedGoogle Scholar
  4. Henle, G. and Henle, W., In “The Epstein-Barr Virus” ( M. Epstein and B. Achong, eds) pp. 297–320. Springer-Verlag, Berlin, 1979.Google Scholar
  5. Kirchner, H., Tosato, G., Blaese, R.M., Broder, S., and Magrath, I.T., Polyclonal immunoglobulin secretion by human lymphocytes exposed to Epstein-Barr virus In vitro. J. Immunol. 122, 1310–13 (1979).PubMedGoogle Scholar
  6. Masucci, M.G., Bejarano, M.T., Masucci, G., and Klein, E., Large granular lymphocytes inhibit the in vitro growth of autologous Epstein-Barr virus-infected B cells. Cell Immunol. 76, 311–321 (1983).PubMedCrossRefGoogle Scholar
  7. Moss, D.J., Rickinson, A.B., and Pope, J.H., Long-term cell mediated immunity to Epstein Barr virus in man. I. Complete regression of virus infected transformation in cultures of seropositive donor leukocytes. Int. J. Cancer 22, 662–668 (1978).Google Scholar
  8. Nilsson, K., Klein, G., Henle, W., and Henle, G., The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBV. Int. J. Cancer 8, 443–450 (1971).PubMedGoogle Scholar
  9. Pope, J.H., Scott, W., and Moss, D.J., Cell relationships in transformation of human leukocytes by Epstein-Barr virus. Int. J. Cancer 14, 122–129 (1974).PubMedCrossRefGoogle Scholar
  10. Rickinson, A.B., Wallace, L.E., and Epstein, M.A., HLA-restricted T-cell recognition of Epstein-Barr virus-infected B cells. Nature 283, 865–867 (1980).PubMedCrossRefGoogle Scholar
  11. Thorley-Lawson, D.A., Chess, L., Strominger, J.I., Suppression of in vitro Epstein-Barr virus infection. A new role for adult human T lymphocytes. J. Exp. Med. 146, 495–508 (1977).PubMedCrossRefGoogle Scholar
  12. Thorley-Lawson, D.A., The transformation of the adult but not newborn lymphocytes by Epstein-Barr virus and phytohemagglutinin is inhibited by interferon: the early suppression by T cells of Epstein-Barr virus infection is mediated by interferon. J. Immunol. 126, 829–833 (1981).PubMedGoogle Scholar
  13. Tosato, G., Magrath, I.T., and Blaese, R.M., T-cell mediated immunoregulation of Epstein-Barr virus (EBV) induced B lymphocyte activation in EBV-seropositive and EBV-seronegative individuals. J. Immunol. 128, 575–579 (1982).PubMedGoogle Scholar
  14. Tosato, G. and Blaese, R.M., Epstein-Barr virus infection and immunoregulation in man. Adv. Immunol., in press.Google Scholar
  15. Yachie, A., Miyawaki, T., Yokoi, T., Nagaochi, T., and Taniguchi, N., la-positive cells generated by PWM stimulation within 0KT4 + subset interact with 0KT8 + cells for inducing active suppression on B cell differentiation in vitro. J. Immunol. 129, 103–106 (1982).PubMedGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • G. Tosato
    • 1
  • S. E. Pike
    • 1
  • R. M. Blaese
    • 2
  1. 1.FDABethesdaUSA
  2. 2.NIHBethesdaUSA

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