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Localization of Epstein-Barr Virus Early Antigen (EA) by Electron Microscopy

  • Masamichi Kishishita
  • Yohei Ito
  • Janos Luka
  • Gary R. Pearson
Part of the Developments in Medical Virology book series (DIMV, volume 1)

Abstract

The localization of polypeptides (50–52,85K) associated with the diffuse and restricted components of early antigen EA in Epstein-Barr virus (EB virus)- infected cells was demonstrated with immune electron microscopy (EM) using colloidal — gold staining. P3HR-1 cells were activated to express the viral antigens by treatment with 4 mM n-butyrate and 20 ng/ml TPA. After 32–38 hrs, the cells were fixed with 2% glutaraldehyde, dehydrated with alcohol and propylene oxide, and embeded in epon. Ultra-thin section were prepared and incubated first with the appropriate monoclonal antibody, then with Staphylococcal protein A — gold, and finally with uranyl acetate. EM examination revealed that the 50–52 K component of EA-D was localized both in nucleoplasm and cytoplasm of EA-positive cells, while the 85 K component of EA-R was located mainly in the cytoplasm, as indicated by specific colloidal-gold staining.

Keywords

Propylene Oxide Osmic Acid Early Antigen Staphylococcal Protein Immune Electron Microscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Masamichi Kishishita
    • 1
    • 2
  • Yohei Ito
    • 1
    • 2
  • Janos Luka
    • 1
    • 2
  • Gary R. Pearson
    • 1
    • 2
  1. 1.Kyoto UniversityJapan
  2. 2.Georgtown UniversityUSA

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