Summary
We investigated the interaction of hydrocortisone (HC) and EBV. The treatment of P3HR-1 cells (propagated at 34°C and 37°C) with various concentrations of HC for 7 and 21 days resulted in enhanced levels of antigen positive cells with a maximum increase at 21 days. Virus harvested from HC-treated P3HR-1 cells grown at 34°C had a 1–2 log higher titer in Raji cells when compared to control virus.
Treatment of AG876 EB virus producer cells grown at 34°C with 5 and 10 μg/ml HC for 7 and 21 days resulted in up to a 3-fold higher level of IF membrane positive cells. Cells treated for 21 days with 10 μg/ml of HC exhibited a 3-5-fold increase in VCA positive cells. When human cord blood mononuclear cells were infected with AG876 EBV and maintained in HC, earlier transformation was observed.
These data suggest that hydrocortisone is able to enhance the expression of the EBV genomes present in human cells and leads to increased levels of antigen expression and virus production. The mechanism by which this glucocorticoid hormone modulates EBV expression remains to be determined.
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Ablashi, D.V., Whitman, J., Dahlberg, J., Armstrong, G., Rhim, J. (1985). Hydrocortisone Enhancement of Both EBV Replication and Transformation of Human Cord Lymphocytes. In: Levine, P.H., Ablashi, D.V., Pearson, G.R., Kottaridis, S.D. (eds) Epstein-Barr Virus and Associated Diseases. Developments in Medical Virology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2625-0_36
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DOI: https://doi.org/10.1007/978-1-4613-2625-0_36
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