Summary
EBV DNA from B95-8 strain was introduced into human cord blood lymphocytes (CBL) using reconstituted Sendai virus envelopes (RSVE) as gene transfer vehicles. The DNA- treated CBL expressed EB virus nuclear antigen (EBNA) and synthesized cellular DNA at an increased rate but were not immortalized. However, when EBV DNA transfer was followed by exposure to UV-in activated virions, full transformation was achieved. The resulting lympho-blastoid cell lines, termed NEB, were of B-cell origin, contained 25–50 EBV genome equivalents/cell, but were not capable of expressing EA or VCA, or releasing infectious virus. Permanent cell lines were also established when CBL were coinfected with purified EBV DNA and EBV of the HR-1 strain. These cells, termed HBD, were of T-cell origin, did not express EBNA, but contained EBV genomes as determined by nucleic acid hybridization. The HBD cells also expressed the early and virus capsid antigens of EBV as determined by immunofluorescence and radio immunoprecipitation. The NEB and HBD cell lines will be useful for analyzing the mechanism of cell transformation by EBV.
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© 1985 Martinus Nijhoff Publishing, Boston
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Volsky, D.J., Volsky, B., Hedeskog, M., Sinangil, F., Gross, T.G. (1985). Transformation of Human Lymphocytes by Coinfection with EBV DNA and Transformation-Defective Virions. In: Levine, P.H., Ablashi, D.V., Pearson, G.R., Kottaridis, S.D. (eds) Epstein-Barr Virus and Associated Diseases. Developments in Medical Virology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2625-0_34
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DOI: https://doi.org/10.1007/978-1-4613-2625-0_34
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