Novel Biological Functions Associated with Epstein-Barr Virus DNA
Transfection of primary kidney cells of non-human primates with specific EBV DNA fragments from a recombinant DNA cosmid library, has allowed two functions associated with the virus to be mapped on to the viral genome. One, designated the “p31 function” is associated with cellular immortalisation, and the other, designated the “p5 function” appears to play a role in cellular differentiation. Transfection experiments with fragment p31 show that in vitro, epithelial cells of both AGMK, marmoset and human origin are selected for growth proliferation in preference to fibroblasts. Poorly differentiated epithelial cell lines have been established. In the presence of EBV DNA fragments p31 + p5, “dome” producing cells from primary marmoset kidneys have been obtained. A working hypothesis regarding EBV-induced transformation of epithelial cells is put forward.
KeywordsTissue Culture Condition And2 IIII Royal Postgraduate Medical School Foetal Human Kidney Nonnal Tissue Culture
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