Novel Biological Functions Associated with Epstein-Barr Virus DNA
Transfection of primary kidney cells of non-human primates with specific EBV DNA fragments from a recombinant DNA cosmid library, has allowed two functions associated with the virus to be mapped on to the viral genome. One, designated the “p31 function” is associated with cellular immortalisation, and the other, designated the “p5 function” appears to play a role in cellular differentiation. Transfection experiments with fragment p31 show that in vitro, epithelial cells of both AGMK, marmoset and human origin are selected for growth proliferation in preference to fibroblasts. Poorly differentiated epithelial cell lines have been established. In the presence of EBV DNA fragments p31 + p5, “dome” producing cells from primary marmoset kidneys have been obtained. A working hypothesis regarding EBV-induced transformation of epithelial cells is put forward.
KeywordsAgar Ouabain Tetradecanoyl
Unable to display preview. Download preview PDF.
- BENNINGTON, J.L. and BECKWITH, J.B. In: H.I. FIMINGER (ed), Tumors of the kidney, renal pelvis and ureter, Atlas of tumor pathology vol. 12, pp 289, 302, Am. Cancer Society and Armed Force Inst. Path. (1979).Google Scholar
- GRIFFIN, B.E. Transformation of cells by Epstein-Barr virus: An hypothesis. In: N,A. Mitchison and M. Feldmann (eds.) 16th Int. Leucocyte Culture Conf., Humana Press Inc., in press.Google Scholar
- GRIFFIN, B.E., KARRAN, L., KING, D., and CHANG, S.E. Immortalising gene(s) encoded by Epstein-Barr virus. In: P.W. Rigfay and N.A. Wilkie (eds). Viruses and Cancer. Soc. Gen. Microbiol., vol. 37, Cambridge Univ. Press in press.Google Scholar
- HANDLER, J.S., PERKINS, F.M. and JOHNSON, J.P. Studies of renal cell function using cell culture techniques. Am, J. Physiol. 238, F1-F9 (1980).Google Scholar
- HSU, T.C. and BENIRSCHKE, K. In: An Atlas of Mammalian Chromosomes, vol. 1, folio 1, pp.46,48. Springer Verlag, Berlin (1967).Google Scholar
- JEANG, K.T. and HAYWARD, S.D. Organization of the Epstein- Barr virus DNA molecule. III. Location of the P3HR-1 deletion junction and characterization of the NotI repeat units that form part of the template for an abundant 12 O-tetradecanoyl phorbol-13-acetate-induced mRNA transcript. J.Virol. 48. 135–148 (1983).PubMedGoogle Scholar
- RABSON, M., GRADOVILLE, L., HESTON, L. and MILLER, G. Non-immortalizing P3J-HR-1 Epstein-Barr virus: A deletion mutant of its transforming parent, Jijoye. J. Virol. 44, 834–844 (1982).Google Scholar