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Epstein-Barr Virus (EBV) Growth Transformation is Associated with an Alteration in c-myc Chromatin Structure

  • William H. Schubach
  • Bart H. Steiner
  • Mark Birkenbach
Part of the Developments in Medical Virology book series (DIMV, volume 1)

Summary

Because the c-myc gene is involved in translocations in Burkitt’s lymphoma, and because of the association of this neoplasm with EBV infection, we have studied the chromatin structure of the c-myc gene in EBV growth-transformed cells and in an isogenic population of cells enriched for B-cells. No correlation between the location of DNaseI-hypersensitive sites and translocation breakpoints in c-myc was found. A novel DNaseI-hypersensitive site appears within the second c-myc intron of growth-transformed cells that is absent in nontransformed cells. In contrast, the pattern of nuclease sensitivity around the pro-α-2-collagen gene is identical in both cells. Thus some gene product encoded or induced in EBV transformed cells may serve to alter chromatin structure in c-myc. This alteration might play a role in controlling c-myc expression in growth-transformed cells.

Keywords

Chromatin Structure Nontransformed Cell Translocation Breakpoint Hypersensitive Site Avian Leukosis Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • William H. Schubach
    • 1
  • Bart H. Steiner
    • 1
  • Mark Birkenbach
    • 2
  1. 1.Department of MedicineSection of Medical OncologyUSA
  2. 2.Department of Laboratory Medicine and PathologyUniversity of MinnesotaMinneapolisUSA

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