Epstein-Barr Virus (EBV) Growth Transformation is Associated with an Alteration in c-myc Chromatin Structure
Because the c-myc gene is involved in translocations in Burkitt’s lymphoma, and because of the association of this neoplasm with EBV infection, we have studied the chromatin structure of the c-myc gene in EBV growth-transformed cells and in an isogenic population of cells enriched for B-cells. No correlation between the location of DNaseI-hypersensitive sites and translocation breakpoints in c-myc was found. A novel DNaseI-hypersensitive site appears within the second c-myc intron of growth-transformed cells that is absent in nontransformed cells. In contrast, the pattern of nuclease sensitivity around the pro-α-2-collagen gene is identical in both cells. Thus some gene product encoded or induced in EBV transformed cells may serve to alter chromatin structure in c-myc. This alteration might play a role in controlling c-myc expression in growth-transformed cells.
KeywordsChromatin Structure Nontransformed Cell Translocation Breakpoint Hypersensitive Site Avian Leukosis Virus
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