The Host-Cell Range of the Epstein-Barr Virus

  • Ronald Glaser
Part of the Developments in Medical Virology book series (DIMV, volume 1)


The Epstein-Barr virus (EBV) has been classified as a B-lymphocyte tropic virus, primarily based on the host-range of EBV under laboratory conditions. Since it is now generally accepted that the EBV is associated with the epithelial cells of NPC, an important issue in the pathogenesis of EBV is the host range of cells that can be infected. Data have accumulated from several laboratories showing that EBV can infect certain epithelial cells directly, including epithelial explant cell cultures prepared from NPC biopsy specimens, epithelial tumor cell lines, normal epithelial nasopharyngeal cells from squirrel monkeys, and, more recently, primary human cervical epithelial cells. In addition, important information has come from studies in which laboratory manipulations have been used to insert EBV or EBV DNA into a variety of cell types. These procedures include transfection, microinjection, and receptor implantation experiments, as well as the preparation of epithelial hybrid cells. The ability to demonstrate routine direct infection of epithelial cells, and more importantly, to demonstrate that they can be transformed by EBV, will help clarify the association of EBV with NPC.


Squirrel Monkey Early Antigen African Green Monkey Kidney Cell Nasopharyngeal Epithelial Cell Epithelial Tumor Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ben-Bassat, H., Mitrani-Rosenbaum, S., and Goldblum, N., Induction of Epstein-Barr virus nuclear antigen and DNA synthesis in a human epithelial cell line after Epstein- Barr virus infection. J. Virol., 41:703–708 (1982).PubMedGoogle Scholar
  2. Boyd, V.A., Stoerker, J., Holliday, J.E., and Glaser, R., Mapping of Epstein-Barr virus antigens by microinjection of human cells. This volume (in press).Google Scholar
  3. Calnek, B.W., Adlinger, H.K., and Kahn, D.E., Feather- follicle epitheliums A source of envelope and infectious cell-free Herpesvirus of Marek′s disease. Avian Pis., 14; 219–233 (1970).CrossRefGoogle Scholar
  4. Desgranges, C., Bornkamm, W., Zeng, Y., Wang, P.C., Zhu, J.S., Shang, M., and de-The, G. Detection of Epstein-Barr viral DNA internal repeats in the nasopharyngeal mucosa of Chinese with IgA-EBV specific antibodies. Int. J. Cancer 29:87–95 (1982).PubMedCrossRefGoogle Scholar
  5. Epstein, M.A., Achong, B.G., and Barr, Y.M., Virus particles in cultured lymphoblasts from Burkitt′s lymphoma. Lancet, 1:702–703 (1964).PubMedCrossRefGoogle Scholar
  6. Glaser, R., and O′Neill, F.J., Hybridization of Burkitt lymphob1astoid cells. Science, 176:1245–1247 (1972).PubMedCrossRefGoogle Scholar
  7. Glaser, R., and Rapp, F., Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells. J. Virol., 10:288–296 (1972).PubMedGoogle Scholar
  8. Glaser, R., Decker B., Farrugia, R., Shows T., and Rapp, F., Growth characteristics of Burkitt somatic cell hybrids in vitro. Cancer Res., 33:2026–2029 (1973a).Google Scholar
  9. Glaser, R., Nonoyama, M., Decker, B., and Rapp, F., Synthesis of Epstein-Barr virus antigens and DNA in activated Burkitt somatic cell hybrids. Virology, 55.:62–69 (1973b).CrossRefGoogle Scholar
  10. Glaser, R., and Nonoyama, M., Host cell regulation of induction of Epstein-Barr virus. J. Virol., 14:174–176, (1974).PubMedGoogle Scholar
  11. Glaser, R., Farrugia, R., and Brown, N., Effect of the host cells on the maintenance and replication of Epstein-Barr virus. Virology, 69:132–142 (1976a).CrossRefGoogle Scholar
  12. Glaser, R., de-The, G., Lenoir, G., and Ho. J.H.C., Superinfection of epithelial nasopharyngeal carcinoma cells with Epstein-Barr virus. Proc. Nat. Acad. Sci., 73:960–963, (1976b).CrossRefGoogle Scholar
  13. Glaser, R., Lenoir, G., Ferrone, S., Pellegrino, M., and de-The, G., Cell surface markers on epithelial/Burkitt hybrid cells superinfected with Epstein-Barr virus. Cancer Res. 37:2291–2296 (1977).PubMedGoogle Scholar
  14. Glaser, R., Lee, K.J., Lang, C.M., and Levy, B., Seroconversion against Epstein-Barr virus in two non-human primate species infected by the oropharyngeal route. J. Infec. Pis. 138:695–698 (1978).CrossRefGoogle Scholar
  15. Glaser, R., Lang, C.M., Lee, K.J., Schuller, D.E., Jacobs, D., and McQuattie, C., Attempt to infect non-malignant nasopharyngeal epithelial cells from humans and squirrel monkeys with Epstein-Barr virus. J. Nat. Cancer Inst., 64: 1085–1090, (1980).PubMedGoogle Scholar
  16. Glaser, R., Boyd, A., Stoerker, J., and Holliday, J., Functional mapping of the Epstein-Barr virus genome: Identification of sites coding for the restricted early antigen, the diffuse early antigen and the nuclear antigen. Virology, 129:188–198 (1983).PubMedCrossRefGoogle Scholar
  17. Glaser, R., Noyes, I., and Milo, G., Pathogenesis of Epstein-Barr virus infection: The Host-Range of EBV now includes epithelial cells. J. Cell. Biochem. (in press).Google Scholar
  18. Graessmann, A., Wolf, H., and Bornkamm, G.W., Expression of Epstein-Barr virus genes in different cell types after microinjection of viral DNA. Proc. Nat. Acad. Sci. USA, 77:433–436 (1980).PubMedCrossRefGoogle Scholar
  19. Griffin, B.E., and Karran, L., Immortalization of monkey epithelial cells by specific fragments of Epstein-Barr virus DNA. Nature, 309:78–82 (1984).PubMedCrossRefGoogle Scholar
  20. Grogan, E., Miller, G., Henle, W., Rabson, M., Shedd, D., and Niederman, J.C., Expression of Epstein-Barr viral early antigen in monolayer tissue cultures after transfection with viral DNA and DNA fragments. J. Virol., 40:861–869 (1981).PubMedGoogle Scholar
  21. Henle, G., Henle, W., and Diehl, V., Relation of Burkitt tumor associated herpes-type virus to infectious mononucleosis. Proc. Nat. Acad. Sci. USA, 59:94–101 (1968).PubMedCrossRefGoogle Scholar
  22. Huang, D.P., Ho, H.C., Ng, M.H., and Lui, M., Possible transformation of nasopharyngeal epithelial cells in culture with Epstein-Barr virus from B95-8 cells. Brit. J. Cancer, 35:630–634 (1977).PubMedCrossRefGoogle Scholar
  23. Khelifa, R., and Menezes, J., Sendai virus envelopes can mediate Epstein-Barr virus binding to and penetration into Epstein-Barr virus receptor-negative cells. J. Virol. 46: 325–322 (1982).Google Scholar
  24. Klein, G., Giovanella, B.C., Lindahl, T., Fialkov, P.J., Singh, S., and Stehlen, J., Direct evidence of the presence of EBV in cells from patients with anaplastic carcinoma of the nasopharynx. Proc. Nat. Acad. Sci. 71:4737–4740 (1974a).CrossRefGoogle Scholar
  25. Lemon, S.M., Hutt, L.M., Shaw, J.E., Li, J-L.H., and Pagano, J.S., Replication of EBV in epithelial cells during infectious mononucleosis. Nature, 268:268–270 (1977).PubMedCrossRefGoogle Scholar
  26. Miller, G., Lisco, H., Kohn, H.I., and Stitt, D., Establishment of cell lines from normal adult human blood leukocytes by exposure to Epstein-Barr virus and neutralization by human sera with Epstein-Barr virus antibody. Proc. Soc. Exp. Biol. Med., 137:1459–1465 (1971).PubMedGoogle Scholar
  27. Miller, G., and Lipman, M., Release of infectious Epstein- Barr virus by transformed marmoset leukocytes. Proc. Nat. Acad. Sci. USA. 70:190–194, (1973).PubMedCrossRefGoogle Scholar
  28. Miller, G., Grogan, E., Heston, L., Robinson, J., and Smith, D., Epstein-Barr viral DNA: Infectivity for human placental cells. Science, 212:452–455 (1981).PubMedCrossRefGoogle Scholar
  29. Saemundsen, A.K., Purtillo, D.T., Sakamoto, K., Sullivan, J.L., Synnerholm, A.C., Hanto, D., Simmons, R., Anvret, M., Collins, R., and Klein, G., Documentation of Epstein-Barr virus infection in immunodeficient patients with life- threatening lymphoproliferative diseases by Epstein-Barr virus complementary RNA-DNA and virus DNA-DNA hybridization. Cancer Res., 41:4237–4242 (1981).PubMedGoogle Scholar
  30. Shapiro, I.M., and Volsky, D.J., Infection of normal epithelial cells by Epstein-Barr virus. Science, 219:1225–1228 (1982).CrossRefGoogle Scholar
  31. Simons, M.J., and Shanmugaratnam, K. (eds.), The Biology of Nasopharyngeal Carcinoma, Report No. 16, U.I.C.C. Technical Report Series, Vol. 71, U.I.C.C., Geneva (1982).Google Scholar
  32. Sixbey, J.W., Vesterinen, E.H., Nedrud, J.G., Raab-Traub, N., Walton, L.A., and Pagano, J.S., Replication of Epstein- Barr virus in human epithelial cells infected in vitro. Nature, 306:480–483 (1983).PubMedCrossRefGoogle Scholar
  33. Sixbey, J.W., Nedrud, J.T., Raab-Traub, N., Hanes, R.A., and Pagano, J.S., Epstein-Barr virus replication in oropharyngeal epithelial cells. N. Eng. J. Med., 310:1225–1230 (1984).CrossRefGoogle Scholar
  34. Stoerker, J., Parris, D., Yajima, Y., and Glaser, R., Pleiotropic expression of Epstein-Barr virus DNA in human epithelial cells. Proc. Nat. Acad. Sci. USA, 78:5852–5855 (1981).PubMedCrossRefGoogle Scholar
  35. Thompson, J.L., Epstein, M.A., Achong, B.G., Chen, J.J., Production of EB virus by normal human nasopharyngeal epithelial cells exposed to the virus in vitro. Ann. Virol. (Inst. Pasteur), 134E:573–579 (1983).CrossRefGoogle Scholar
  36. Volsky, D.J., Shapiro, I.M., and Klein, G., Transfer of Epstein-Barr virus receptors to receptor-negative cells permits virus penetration and antigen expression. Proc. Nat. Acad. Sci. 77:5453–5457 (1980).PubMedCrossRefGoogle Scholar
  37. Wolf, H., zur Hausen, H., and Becker, V., EBV virus genomes in epithelial nasopharyngeal carcinoma cells. Nature New Biol., 244:245–247, (1973).PubMedGoogle Scholar
  38. Wolf, H., Hause, M., and Wilms, E., On the viral etiology of carcinomas of Waldeyer′s Ring. In Nasopharyngeal Carcinoma: Current Concepts, Prasad, U., Ablashi, D.V., Levine, P.H., and Pearson, G.R. (eds.), University of Malaysia, Kuala Lumpur, p. 277–279 (1983).Google Scholar
  39. zur Hausen, H., Schulte-Holthausen, H., Klein, G., Henle, W., Henle, G., Clifford, P.,and Santesson, L., EB virus DNA in biopsies of Burkitt tumors and anaplastic carcinomas of the nasopharynx. Nature, 228:1056, (1970).PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Ronald Glaser
    • 1
  1. 1.College of Medicine and Comprehensive Cancer CenterThe Ohio State UniversityColumbusUSA

Personalised recommendations