The Host-Cell Range of the Epstein-Barr Virus
The Epstein-Barr virus (EBV) has been classified as a B-lymphocyte tropic virus, primarily based on the host-range of EBV under laboratory conditions. Since it is now generally accepted that the EBV is associated with the epithelial cells of NPC, an important issue in the pathogenesis of EBV is the host range of cells that can be infected. Data have accumulated from several laboratories showing that EBV can infect certain epithelial cells directly, including epithelial explant cell cultures prepared from NPC biopsy specimens, epithelial tumor cell lines, normal epithelial nasopharyngeal cells from squirrel monkeys, and, more recently, primary human cervical epithelial cells. In addition, important information has come from studies in which laboratory manipulations have been used to insert EBV or EBV DNA into a variety of cell types. These procedures include transfection, microinjection, and receptor implantation experiments, as well as the preparation of epithelial hybrid cells. The ability to demonstrate routine direct infection of epithelial cells, and more importantly, to demonstrate that they can be transformed by EBV, will help clarify the association of EBV with NPC.
KeywordsSquirrel Monkey Early Antigen African Green Monkey Kidney Cell Nasopharyngeal Epithelial Cell Epithelial Tumor Cell Line
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