An EBV RNA with A Repetitive Spliced Structure
We are studying the Epstein-Barr virus genes expressed in the Burkitt’s lymphoma latently infected Raji cells. We describe here a cDNA representing a spliced RNA transcribed rightward from the IR1-U2 region. The cDNA contains several repeats of two exons, 66 and 132 bp, which are transcribed from the IR1 repeats, and four exons transcribed from U2. The longest open reading frame of the cDNA presumably corresponds to the carboxy-terminal 261 amino acids of a polypeptide containing several repeats of a 66 amino acid sequence. Since part of this coding region is deleted in the P3HR-1 non-immortalizing virus, this polypeptide might be involved in the process of growth-transformation of B-lymphocytes.
KeywordsHydrolysis Phenol Lymphoma Codon EDTA
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- HAYWARD, S., LAZAROWITZ, S., and HAYWARD, G., Organization of the Epstein-Barr virus DNA molecule II: fine mapping of the boundaries of the internal repeat cluster of B95-8 and identification of additional small tandem repeats adjacent to the HR-1 deletion. J. Virol., 43, 201–212 (1982).PubMedGoogle Scholar
- HELLER, M., VAN SANTEN, V., and KIEFF, E., Simple repeat sequence in Epstein-Barr virus DNA is transcribed in latent and productive infections. J. Virol., 44, 311–320 (1982 b).Google Scholar
- JEANG, K., and HAYWARD, S., Organization of the Epstein- Barr virus DNA molecule III: location of the P3HR-1 deletion junction and characterization of the Not I repeat units that form part of the template for an abundant 12- 0-tetradecanoylphorbol-13-acetate-induced mRNA transcript. J. Virol., 48, 135–148 (1983).PubMedGoogle Scholar
- JONES, M., FOSTER, L., SHEEDY, T., and GRIFFIN, B., The EB virus genome in Daudi Burkitt′s lymphoma cells has a deletion similar to that observed in a non-transforming strain (P3HR-1) of the virus. The EMBO J., 3, 813–821 (1984).Google Scholar
- KIEFF, E., DAMBAUGH, T., HUMMEL, M., and HELLER, M., Epstein-Barr virus transformation and replication. In: Klein G. (ed.), Advances in viral oncology, pp. 133–182, Raven Press, New York (1983).Google Scholar
- THOMAS-POWELL, A., KING, W., AND KIEFF, E., Epstein-Barr virus-specific RNA III: mapping of DNA encoding viral RNA in restringent infection. J. Virol., 29, 261–274 (1979).Google Scholar