An EBV RNA with A Repetitive Spliced Structure
We are studying the Epstein-Barr virus genes expressed in the Burkitt’s lymphoma latently infected Raji cells. We describe here a cDNA representing a spliced RNA transcribed rightward from the IR1-U2 region. The cDNA contains several repeats of two exons, 66 and 132 bp, which are transcribed from the IR1 repeats, and four exons transcribed from U2. The longest open reading frame of the cDNA presumably corresponds to the carboxy-terminal 261 amino acids of a polypeptide containing several repeats of a 66 amino acid sequence. Since part of this coding region is deleted in the P3HR-1 non-immortalizing virus, this polypeptide might be involved in the process of growth-transformation of B-lymphocytes.
KeywordsBurkitt Lymphoma Raji Cell Longe Open Reading Frame PstI Restriction Site Incomplete Copy
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