Abstract
Sarcolemmal integrity may be subjected to multiple metabolic perturbations during myocardial ischemia. In the present study, sarcolemma were exposed in vitro to high Ca2+ concentrations, lipolytic enzymes, amphiphilic lipid metabolites and drugs to assess the response of sarcolemmal structural and functional endpoints to these conditions. It was found that significant production of lysophospholipids could result from addition of Ca2+ to sarcolemmal membranes. Furthermore, exogenous mammalian lipases were able to differentially hydrolyze specific membrane phospholipids. Finally, the addition of exogenous amphiphilic lipids and drugs to sarcolemma both stimulated and inhibited the Na+,K+-ATPase depending upon the specific conditions. Thus, these in vitro manipulations provide a model system for assessment of sarcolemmal injury and the testing of pharmacological agents under conditions where metabolites can be altered in highly controlled fashion.
Supported by National Heart, Lung and Blood Institute Grants T32-HL-07548 and R01 HL-28985.
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© 1985 Martinus Nijhoff Publishing, Boston
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Weglicki, W.B., Kramer, J.H., Franson, R.C., Pang, D.C., Owens, K. (1985). Perturbations of Sarcolemma by Lipases and Amphiphiles. In: Stone, H.L., Weglicki, W.B. (eds) Pathobiology of Cardiovascular Injury. Developments in Cardiovascular Medicine, vol 49. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2621-2_19
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DOI: https://doi.org/10.1007/978-1-4613-2621-2_19
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