Abstract
MAM-6 is an antigen present in almost all carcinomas. It is defined by five different monoclonal antibodies binding to five different epitopes present on the carbohydrate moiety of a glycoprotein. In human skim milk MAM-6 is a complex glycoprotein containing sialic acids and of an apparent molecular weight in SDS polyacrylamide gels of over 400Kd. A sandwich-radioimmunoassay has been developed to detect MAN-6 in serum of healthy persons, patients with benign breast tumors, malignant breast cancer and patients with other diseases. The average NAM-6 level present in serum of 43 healthy non smokers was 2.3 m.u./ml while the average level in healthy smokers was slightly enhanced to 3.1 m.u./ml. In sera of benign tumor patients similar levels were found, but increased levels of 5 m.u./ml and over were found in respectively 18, 43, 51, and 85% of the sera of stage I, II, III, IV breast cancer patients. MAM-6 levels correlated with the tumor load of the patients and may be used to monitor the course of the disease during therapy.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Taylor-Papadimitriou, J., J. Peterson, J. Arklie, J. Burchell, R.L. Ceriani and W.F. Bodmer. Int. J. Cancer 28: 17–21, 1981.
Colcher, D., P. Hand, M. Nutti and J. Schlom. Proc. Natl. Acad. Sci. (Wash.) 78: 3199–3203, 1981.
Ceriani, R.L., J.A. Peterson, J.Y. Lee, R. Moncada and E.W. Blank. Somatic Cell Genetics 9: 415–427, 1983.
Foster, C.S., E.A. Dinsdale, P.A.W. Edwards, and A.M. Neville. Virch. Arch. (Path. Anat.) 394: 295–305.
Menard, S., E. Tagliabue, S. Canevari, G. Fossati and M.I. Colnaghi. Cancer Res. 43: 1295–1300.
Papsidero, L.D., G.A. Groghan, M.J. O’Connell, L.A. Valenzuela, T. Nemoto and T.M. Chu. Cancer Res. 43: 1741–1747, 1983.
Soule, R.S., E. Linder and T.S. Edgington. Proc. Natl. Acad. Sci. (Wash.) 80: 1332–1336, 1983.
Hilkens, J., J. Hilgers, F. Buijs, P.H. Hageman, D. Schol, G. van Doornewaard and J. van den Tweel. In: Protides of the Biological Fluids 31, H. Peeters, ed., Pergamon Press, 1984.
Hilkens, J., F. Buijs, J. Hilgers, P.H. Hageman, A. Sonnenberg, J. Calafat and M.A. van der Valk. Int. J. Cancer 34: 197–206, 1984.
De Graaf, P., Ph.C. Hageman, J. Hilgers, J.G.W. Hilkens and J.L. Peterse. Ned. Tijdschr. Geneeskd. 127: 1715–1721, 1983.
Haisma, H., W. Goedemans, M. de Jong, J. Hilkens, J. Hilgers, H. Dullens and W. den Otter. Cancer Immunol. Immunother. 17: 62–65, 1984.
Hilkens, J., V. Kroezen, H. Bonfrer, P. Bruning, J. Hilgers and M. van Eijkeren. In: Protides of the Biological Fluids 31, H. Peeterse, ed., Pergamon Press, 1984c.
Ouchterlony, O. Acta Path. Microbiol. Scand. 25: 186, 1948.
Fraker, P.J. and J.C. Speck. Biochem. Biophys. Res. Commun. 80: 849–857, 1978.
Simeral, L.S., W. Kapmeyer, W.P. MacConnel, and N.O. Kaplan. J. Biol. Chem. 255: 11098–11101, 1980.
Ey, P.L., S.J. Prowse and C.R. Jenkin. Immunochem. 15: 429–436, 1978.
Laemmli U.K. Nature 227: 680–685, 1970.
Bast, R.C., T.L. Klug, E. St. John, E.E. Jenison, J.M. Niloff, H. Lazerus, R.S., Berkowitz, T. Leavitt, C.T. Griffith, L. Parker, V.R. Zurawski and R.C. Knapp. J. Med. 309: 883–887, 1983.
Burchell, J., H. Durbin and J. Taylor-Papadimitriou. J. Immmunol. 131: 508–513, 1983
Shimizu, M. and K. Yamauchi. J. Biochem. 9: 515–524.
Klug, T.L., R.C. Bast, J.M. Niloff, R.C. Knapp and V.R. Zurawski. Cancer Res. 44: 1048–1053, 1984.
Morgan, A.C., M.M. Crane and R.D. Rosen. J. Natl. Cancer Inst. 72: 243–248, 1984.
Giacomini, P., A.K. Ng, R.R.S. Kantor, P.G. Natali and S. Ferrone. Cancer Res. 44: 1048–1053, 1983.
Uotila, M., E. Ruoslahti and E. Engvall. J. Immunol. Methods 42: 11–15, 1981.
DelVillano, B.C., S. Brennan, P. Brock, C. Bucher, V. Liu, M. McClure, B. Rake, S. Space, B. Westrick, H. Schoemaker and V. Zurawski. Clin. Chem. 29: 549–552, 1983.
Ceriani, R.L., M. Sasaki, H. Sussman, W.M. Wara and E.W. Blank. Proc. Natl. Acad. Sci. USA 79: 5420–5424, 1982.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1985 Martinus Nijhoff Publishing, Boston
About this chapter
Cite this chapter
Hilkens, J. et al. (1985). MAM-6, a Carcinoma Associated Marker: Preliminary Characterisation and Detection in Sera of Breast Cancer Patients. In: Ceriani, R.L. (eds) Monoclonal Antibodies and Breast Cancer. Developments in Oncology, vol 35. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2617-5_3
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2617-5_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9637-9
Online ISBN: 978-1-4613-2617-5
eBook Packages: Springer Book Archive