Monoclonal Antibodies Against Estrogen Receptor: Specificity, Sensitivity and Potential Applications

  • K. McCartyJr.
  • J. Snowhite
  • E. Cox
  • L. Miller
  • K. McCartySr.
Part of the Developments in Oncology book series (DION, volume 35)

Abstract

A study of immunohistochemical localization of estrogen receptor in one hundred and thirty-four human breast cancers and twenty-three metastatic lesions shows sensitivities and specificities of 91% as compared to biochemical analyses. A close correlation between the categorization of tumors by both methods is demonstrated. The immunohistochemical assay is shown to be sensitive and specific at threshold values of receptor. A comparison to clinical response for an initial cohort of twenty-three patients shows excellent predictive value for the immunohistochemical method.

Keywords

Formalin Sucrose Acetone Estrogen EDTA 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    King, W.J., Greene, G. L.: Nature 307: 745, 1984.PubMedCrossRefGoogle Scholar
  2. 2.
    Silva, J.S., Cox, C.E., Wells, S.A.; et.al.: Surgery, 92: 443, 1982.PubMedGoogle Scholar
  3. McCarty, K.S., Jr., Lubahn, D.B., McCarty, K.S., Sr.: Clinics in Endocrinology and Metabolism 12: 133, 1983.PubMedCrossRefGoogle Scholar
  4. 4.
    Panko, W.B., Mattioli, C.A., Whealer’ T.M.: Cancer 49: 2148, 1982.PubMedCrossRefGoogle Scholar
  5. 5.
    Nicholson, R.I., Campbell, F.C., Blarney, R.W., et al. J. Steroid Biochemistry 15: 193, 1981.CrossRefGoogle Scholar
  6. 6.
    Byar, D.P., Searer, M.E., McGuire, W.L.: Eur. J. Cancer Clin. Oncol. 15: 299, 1979.Google Scholar
  7. 7.
    Jensen, E:V., Greene, G.L., Closs, L.E., DeSombre, E.V., and Nadji, M.: Rec. Prog. Horm. Res. 38: 1, 1982.PubMedGoogle Scholar
  8. 8.
    Greene, G.L., Jensen, E.V.: J. Stér id Biochem. 16: 353, 1982Google Scholar
  9. 9.
    Pertschuk, L.P., Eizenberg, K.B., Carter, A.C., et al: Breast Cancer Res. and Treat. 3: Abstract 304, 1983.Google Scholar
  10. 10.
    Silva, J.S., Georgiade, G.S., Dilley, W.G., et al.: Cancer 51: 1297, 1982.CrossRefGoogle Scholar
  11. 11.
    McCarty, K.S., Jr., Woodard, B.H., Nichols, D.E., et. al.: Cancer 46: 2842, 1980.PubMedCrossRefGoogle Scholar
  12. 12.
    Greene, G.L.: Methods in Enzymology, Academic Press. In Press, 1984.Google Scholar
  13. 13.
    Miller, L.S., Tribby, I.E., Miles, M.R., Tamita, J.T., Nolan, C.: Fed. Proc. 41: Abstr. 1459 (1982).Google Scholar
  14. 14.
    Greene, G.L.: Roy; AK, Clark, JH (eds.) Springer Verlag, pp. 191, 1983.Google Scholar
  15. 15.
    Lubahn, D.B., Ph.D. Dissertation, Duke University, Sept, 1983.Google Scholar
  16. 16.
    Jensen, E.V., Jacobson, H.I.: In Biologic Activity ofSteroids in Relation to Cancer, Pincus, G. and Völlmer,., s., ca emic ress, ew York, 1960, 161.Google Scholar
  17. 17.
    Jensen, E.V., Jacobson, H.I. Rec. Prog. Hormone Res 18: 387, 1962.Google Scholar
  18. Galen, R.S., Gambino, S.R., Be and Normality: The Predictive Value and Efficiency oo e~ ica is nos1s, Johnwi ey an.. ons, nc.Google Scholar
  19. 19.
    McGuire, W.L., Vollmer, E.P. Carbone, P.P., Eds.: Raven Press, New York, 1975.Google Scholar
  20. 20.
    Welshons, W.V, Liebermen, M.E., Gorski, J.: Nature 307: 747, 1984PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • K. McCartyJr.
    • 1
  • J. Snowhite
    • 1
  • E. Cox
    • 1
  • L. Miller
    • 1
  • K. McCartySr.
    • 1
  1. 1.Duke UniversityDurhamUSA

Personalised recommendations