Abstract
We have showed that mononuclear cells of patients with primary immunodeficiency displayed a remarkable scattering of surface enzyme activities, whereas normal peripheral blood lymphocytes and non proliferating cells of patients with recurrent infections of the respiratory tract did not [1, 2]. Almost complete lack of correlations between ectoenzyme activities was also noted in immunodeficient cells, in contrast to a broad correlation level in control lymphocytes [1]. On this basis, an hypothesis was developed suggesting a dynamic rearrangements of integral and associated constituents of the plasma membrane in acute pathologic situations leading to a discrete enzyme expression. This view was strenghtened by an enzymatic analysis of blood and reticuloendothelial cell malignancies. Specific activity changes of the ectoenzymes were recorded in cells isolated from patients with acute lymphoblastic leukemia. Cellular immunologic phenotype was defined by conventional surface and cytochemical markers [3, 4]. The contribution of the surface enzyme analysis to the identification of differentiation stage and cell origin has been clearly documented by a recent report [5] showing that acute undifferentiated leukemia (AUL; Ia+, cALLA-) could be distinguished into three groups owing to the expression of the plasma membrane enzyme γ-glutamyltranspeptidase (Glupase) and the soluble terminal transferase (TdT). Thus, a leukemic population at a given stage of differentiation expresses a surface immunologic phenotype accompanied by biochemical characteristics of their plasma membrane.
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References
Losa G, Morell A and Barandun S (1982). Correlations between enzymatic and immunologic properties of human peripheral blood mononuclear cells. Amer J Path 107: 191–201.
Maestroni G and Losa G (1984). Clinical and immunobiological effects of an orally administered bacterial extract. Int J Immunopharmacol 6: 111–117.
Morell A, Carrel S, Von Fliedner V et al. (1983). Expression of immunological markers and of ectoenzyme activities on leukemic cells. Verh Dtsch Ges Path 67: 517–521.
Losa G (1982). Enzymatic properties in pericellular membranes of leukemic cells. Adv Exp Med Biol 145: 377–384.
Heumann D, Grob JP, Von Fliedner V and Losa G (1984). Activity of membrane associated y-glutamyltranspeptidase in acute leukemia. Proc. Second International Conference on Malignant Lymphoma, Lugano: 103.
Losa G and Morell A (1980). Enzyme profiles in plasma membrane of human leukemic cell cultures. Eur J Cell Biol 22: 535.
Resch K, Loracher A, Mähler B et al. (1978). Functional mosaicism of the lymphocyte plasma membrane. Biochim Biophys Acta 511: 176–193.
Anderson L, Gahmberg C, Kimura A and Vigzell H(1978). Activated human T-lymphocytes display new surface glycoproteins. Proc Nat Acad Sc USA 75: 3455–3458.
Sachs DH, Kiszkiss P and Kim KJ (1980). Release of la antigens by a cultured B-cell line. J Immunol 124: 2130–2135.
Losa G and Weibel E (1983). Freeze fracture and enzymatic analysis of membrane vesicles in subcellular fractions of rat liver. Cell Molec Biol 29: 129–137.
Conti A and Losa G (1984). Organizzazione biochimica ed ultrastrutturale delle membrane di cellule leucemiche umane coltivate in vitro. Boll Soc Tic Sc Nat LXXI.
Liepins A and Hillman A (1981). Shedding of tumor cell surface membranes. Cell Biol Int Rep 5: 15–26.
De Broe M, Wieme R, Logghe G and Roels F (1977). Spontaneous shedding of plasma membrane fragments by human cells in vivo and in vitro. Clin Chim Acta 81: 237–245.
Dainiak N Cohen C (1982). Surface membrane vesicles from mononuclear cells stimulate erythroid stem cells to proliferate in culture. Blood 60: 583–594.
Carrel S, Buchegger F, Heumann D et al. (1984). Detection of the common acute leukemia antigen (cALLA) in the serum of leukemia patients. (Submitted for publication).
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© 1985 Martinus Nijhoff Publishers, Boston
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Losa, G.A. (1985). Enzymatic and ultrastructural properties of the plasma membrane in human leukemias, in non-Hodgkin’s lymphomas and in human lymphoblastoid cells. In: Cavalli, F., Bonadonna, G., Rozencweig, M. (eds) Malignant Lymphomas and Hodgkin’s Disease: Experimental and Therapeutic Advances. Developments in Oncology, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2607-6_19
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DOI: https://doi.org/10.1007/978-1-4613-2607-6_19
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