Arachidonic Acid Metabolism in the Skin

  • Vincent A. Ziboh
Part of the Prostaglandins, Leukotrienes, and Cancer book series (PLAC, volume 3)


The prostaglandins and related arachidonate metabolites are not normally synthesized or stored to any extent in body tissues. Increased levels are brought about by rapid biosynthesis in response to a variety of chemical, physical and hormonal factors. This synthesis or arachldonlc acid cascade is complex and includes several enzymes that are affected by endogenous and/or exogenous regulatory factors. Generally, molecular oxygen is inserted into a polyunsaturated fatty acid with acyl chain rearrangement to form the various structures. The principle end products of the various branches of the pathway are the prostaglandins, the thromboxanes, prostacyclin, the hydroperoxy and hydroxyfatty acids, and the leukotrlenes. All are derived from the essential fatty acids in the diet.


Arachidonic Acid Essential Fatty Acid Arachidonic Acid Metabolism Hairless Mouse Psoriatic Plaque 
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  1. 1.
    Burr GO, Burr MM: A new deficiency disease produced by the rigid exclusion of fat from the diet. J Biol Chem (82) 345–367, 1929.Google Scholar
  2. 2.
    Burr GO, Burr MM: On the nature and role of fatty acids essential in nutrition, J Biol Chem (86) 587–621, 1930.Google Scholar
  3. 3.
    Vroman HE, Nemeek RA and Hsia SL: Synthesis of lipids from acetate by human preputial and abnominal skin in vitro. J Lipid Res (10) 507–514, 1969.PubMedGoogle Scholar
  4. 4.
    Gray GM and Yardley HJ: Lipid composition of cells isolated from pig, human and rat epidermis. J Lipid Res (16) 434–440, 1975.PubMedGoogle Scholar
  5. 5.
    Ziboh VA and Lord JT: Phospholipase A activity in the skin: Modulators of arachidonic acid release from phosphatidylcholine. Biochem J (184) 283–290, 1979.PubMedGoogle Scholar
  6. 6.
    Galey CI, Ziboh VA, Marcelo CL and Yoorhees JJ: Activation of phospholipase A and C in murine keratinoctyes by phorbol ester 12-0-tetradecanoylphorbol-13-acetate. J Invest Dermatol (80) 345, 1983.Google Scholar
  7. 7.
    Jessup SJ, McDonald-Gibson WJ, Ramwell PJ and Shaw JE: Biosynthesis and release of prostaglandins on hormonal treatment of frog skin and their effect on ion transport. Fed Proceedings (29) 387, 1970.Google Scholar
  8. 8.
    Ziboh VA and Hsis SL: Prostaglandin E2: Biosynthesis and effects on glucose and lipid metabolism in rat skin. Arch Biochem Biophys (146) 100–109, 1971.PubMedCrossRefGoogle Scholar
  9. 9.
    Van Dorp D: Recent developments in the biosynthesis and the analysis of prostaglandins. Annals of the New York Academy of Science (180) 181–199, 1971.Google Scholar
  10. 10.
    Greaves MW and McDonald-Gibson W: Effect of non-steroid anti-inflam-matory drugs on prostaglandin biosynthesis by skin. Brit J Dermatol (88) 47–50, 1973.CrossRefGoogle Scholar
  11. 11.
    Wilkinson DI and Walsh JT: Prostaglandin biosynthesis in the epidermis and dermis of young mouse skin and the effects on calcium and cyclic nucleotides. J Invest Dermatol (68) 210–214, 1977.PubMedCrossRefGoogle Scholar
  12. 12.
    Ruzicka T and Printz MP: Arachidonic acid metabolism in guinea pig skin. Biochem Biophys Acta (711) 391–397, 1982.PubMedGoogle Scholar
  13. 13.
    Jonsson DC, Angaard E: Biosynthesis and metabolism of prostaglandin E2 in human skin. Scan J Clin Lab Invest (29) 289–296, 1972.CrossRefGoogle Scholar
  14. 14.
    Ziboh VA: Biosynthesis of prostaglandin E2 in human skin: Subcellular localization and inhibition of unsaturated fatty acids and anti-infiammatory drugs. J Lipid Res (14) 377–384, 1973.PubMedGoogle Scholar
  15. 15.
    Hammarstrom S, Hamberg M, Samuelsson B, Duell EA, Strawiski M, Voorhees JJ: Increased concentration of non-esterified arachidonic acid. 12L-hydroxy-5,8,10,12-eicosatetraenoic acid, prostaglandin E2 and prostaglandin F2α in epidermis of psoriasis. Proc Nat Acad Sci (72) 5130–5132, 1975.PubMedCrossRefGoogle Scholar
  16. 16.
    Hammarstrom S, Lingren JA, Marcelo C, Duell EA, Anderson RF and Voorhees JJ: Arachidonic acid transformations in normal and psoriatic skin. J Invest Dermatol (73) 180–183, 1979.PubMedCrossRefGoogle Scholar
  17. 17.
    Forstrom L, Goldyne ME and Winklemann RK: Prostaglandin production by human epidermal cells in vitro: A model for studying pharmacological inhibition of prostaglandin synthesis. Prostaglandins (8) 107–113, 1974.PubMedCrossRefGoogle Scholar
  18. 18.
    Fischer SM and Slaga TJ: Modulation of prostaglandin synthesis and tumor promotion. In: Proc. Internati. Cong. Prostaglandins and cancer. Alan R. Liss, N.Y., 1982, pp 255–264.Google Scholar
  19. 19.
    Ziboh VA, Marcelo C and Voorhees JJ: Induced lipoxygenation of arachidonic acid in mouse epidermal keratinocytes by calcium ionophore, A23187. J Invest Dermatol (76) 307, 1981.Google Scholar
  20. 20.
    Brian SD, Camp RDR, Leigh IM and Fordtlutchinson AW: The synthesis of leukotriene B4-like material by cultured human keratinocytes. J Invest Dermatol (78) 328, 1982.Google Scholar
  21. 21.
    Ziboh VA, Casebolt T, Marcelo CL and Voorhees JJ: Enhancement of 5-lipoxygenase activity in soluble preparations of human psoriatic plaque preparation. J Invest Dermatol (80) 359, 1983.Google Scholar
  22. 22.
    Brain S, Camp R, Dowd P, Black AK, Woolard PM, Mallet AI and Greaves MW: Psoriasis and Leukotriene B4. Lancet (2) 762–763, 1982.PubMedCrossRefGoogle Scholar
  23. 23.
    Camp R, Mallet AI, Woolard PM, Brain S, Black AK and Greaves MW: Monohydroxy metabolites of arachidonic acid and linoleic acids in psoriatic skin. J Invest Dermatol (80) 359, 1983.Google Scholar
  24. 24.
    Camp RD and Greaves MW: The catabolismi of prostaglandins by rat skin.Biochem J (186) 153–160, 1980.PubMedGoogle Scholar
  25. 25.
    Ziboh VA, Lord JT and Penneys NS: Alterations of prostaglandin E2-9-ketoreductase activity in proliferating skin. J Lipid Res (18) 37–43, 1977.PubMedGoogle Scholar
  26. 26.
    Mui MM, Hsia SL and Halprin KM: Further studies on adenyl cyclase in psoriasis. Brit J Dermatol (92) 255–262, 1975.CrossRefGoogle Scholar
  27. 27.
    Adachi K, Yoshikawa K, Halprin KM and Levine U: Prostaglandin and cAMP in epidermis. Evidence for the independent action of prostaglandins and adrenaline on the adenyl cyclase system of pig and human epidermis, normal and psoriatic. Brit J Dermatol (92) 381–388, 1975.CrossRefGoogle Scholar
  28. 28.
    Ilzuka H, Adachi K, Halprin KM and Levine V: Adenosine and adenine nucleotides stimulation of skin (epidermal) adenyl cyclase. Biochem Biophys Acta (444) 685–693, 1976.Google Scholar
  29. 29.
    Wilkinson DI and Orenberg EK: Effect of prostaglandins on cyclic nucleotide levels in cultured keratinocytes. Prostaglandins (17) 419–429, 1979.PubMedCrossRefGoogle Scholar
  30. 30.
    Kirscher CW: Effects of specific prostaglandins on development of chick embryo skin and down feather organ in vitro. Develop Biol (16) 203–215, 1967.CrossRefGoogle Scholar
  31. 31.
    Kirscher CW: Accelerated maturation of chick embryo skin treated with a prostaglandin — 278 (PGB): An electron microscopy study. Am J Anat (124) 491–512, 1969.CrossRefGoogle Scholar
  32. 32.
    Ziboh VA, Vanderhoek JY, Lands EM: Inhibition of sheep vesicular gland oxygenase by unsaturated fatty acids from skin of essential fatty acid deficient rats. Prostaglandins (5) 233–239, 1974.PubMedCrossRefGoogle Scholar
  33. 33.
    McCullough JL, Schreiber SH and Ziboh VA: Cell proliferation kinetics of epidermis in the essential fatty acid deficient rat. J Invest Dermatol (70) 318–320, 1978.PubMedCrossRefGoogle Scholar
  34. 34.
    Lowe NJ and Stoughton RB: Essential fatty acid deficient hairless mouse: A model of chronic epidermal hyperproliferation. Brit J Dermatol (96) 155–162, 1977.CrossRefGoogle Scholar
  35. 35.
    Nguyen TT, Ziboh VA, Uematsu S, McCullough JL and Weinstein G: New Model of Scaling Dermatosis: Induction of hyperproliferation in hairless mice with eicosa-5,8,11-trienoic acid. J Invest Dermatol (76) 384–387, 1981.PubMedCrossRefGoogle Scholar
  36. 36.
    Pennys NS, Ziboh VA, Lord JT and Simon P: An inhibitor of prostaglandin synthesis in psoriatic plaque. Nature (254) 351–352, 1975.CrossRefGoogle Scholar
  37. 37.
    Kuehl FA, Humes JL, Tarnoff J, Cirillo VJ and Ham EA: Prostaglandin receptor site: Evidence for an essential role in the action of luteinizing hormone. Science (169) 883–886, 1970.PubMedCrossRefGoogle Scholar
  38. 38.
    Saez JM, Dazord A and Gallet D: ACTH and prostaglandin in human adrenocortical tumors. Apparent modification of a specific component of the ACTH-binding site. J Clin Invest (56) 536–547, 1975.PubMedCrossRefGoogle Scholar
  39. 39.
    Rao CV: Receptors of prostaglandins and gonadotropins in cell membranes of bovine corpus luteum. Prostaglandins (4) 567–576, 1973.CrossRefGoogle Scholar
  40. 40.
    Lefkowitz RJ, Mullikin D, Wood CL, Gore TB, Mukherjee C: Regulation of prostaglandin receptors by prostaglandins and quanine nucleotides in frog erythrocytes. J Biol Chem (252) 5295–5303, 1977.PubMedGoogle Scholar
  41. 41.
    Lord JT, Ziboh VA and Warren SK: Specific binding of prostaglandins E2 and Fα by membrane preparations from rat skin. Endocrinology (102) 1300–1309, 1978.PubMedCrossRefGoogle Scholar
  42. 42.
    Lord JT, Ziboh VA, McCullough JL: Alteration of specific binding of prostaglandin F by hyperproliferative skin membrane fractions from essential fatty acid-deficient rats. Prostaglandins and Medicine (2) 21–31, 1979.PubMedCrossRefGoogle Scholar
  43. 43.
    Lupulescu AP: Autoradiographic studies of 3H-Prostaglandin F into nuclei of epidermal neoplastic cells. J Invest Dermatol (72) 279, 1979.Google Scholar
  44. 44.
    DeAsua, Clingan L and Rudland PS: Initiation of cell proliferation in cultured mouse fibroblasts by prostaglandin F Proc Nat Acad Sci (72) 2724–2728, 1975.PubMedCrossRefGoogle Scholar
  45. 45.
    Lord JT and Ziboh YA: Specific binding of prostaglandin E2 to membrane preparations from human skin: receptor modulation by UYB-irradiation and chemical agents. J Invest Dermatol (73) 373–377, 1979.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Vincent A. Ziboh
    • 1
  1. 1.University of CaliforniaDavisUSA

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