Summary
The interactions between Rous Sarcoma virus RNA and the viral proteins have been analysed in the virus and in vitro. We show that it is protein P12 and not P19 that binds tightly to RSV RNA both in the virus and in vitro. Specific RNA sequences are recognized by protein P12 in the virus and such sequences are close to or involve the splice sites, and the dimer linkage site likely to be required for viral packaging. In vitro P12 does not impair proviral DNA synthesis whereas it can completely inhibit RNA translation. Such data suggest that P12 may control negatively viral RNA splicing and translation and positively the packaging process.
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Abbreviations
- RSV:
-
Rous sarcoma virus
- PAGE:
-
olyacrylamide gel electrophoresis
- UV:
-
ultraviolet light
- nt:
-
nucleotides
- RNP:
-
ribonucleoprotein
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© 1985 Martinus Nijhoff Publishing, Boston
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Darlix, JL., Meric, C., Spahr, PF. (1985). The Interactions of Viral Proteins with Rous Sarcoma Virus RNA and Possible Control of Reverse Transcription, Translation and Virion Assembly. In: Becker, Y., Hadar, J. (eds) Viral Messenger RNA. Developments in Molecular Virology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2585-7_17
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DOI: https://doi.org/10.1007/978-1-4613-2585-7_17
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