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A Short Segment of Viral DNA is Required for the Stimulation of Transcription of Mouse Mammary Tumor Virus Genes by Glucocorticoid Hormones

  • Chapter
Viral Messenger RNA

Part of the book series: Developments in Molecular Virology ((DMVI,volume 7))

Summary

Transcription of mouse mammary tumor virus (MMTV) DNA is stimulated by glucocorticoid hormones in vivo and in mammary tumor cells in culture. We have shown that cloned exogenous and endogenous proviral DNA is expressed in transfected mouse and mink cells. This expression is hormonally controlled and the regulation takes place on the level of initiation of transcription. The element responsible for the glucocorticoid stimulation was first localized in the long terminal repeat (LTR) region of the viral DNA. To more closely locate the important sequences we constructed a plasmid containing the LTR in front of the coding region of the herpes simplex thymidine kinase gene from which the promoter had been eliminated. Specific portions of the LTR were removed and the deleted molecules were tested for their ability to respond to glucocorticoid hormones after transfection into Ltk- aprt- cells. These experiments demonstrated that the DNA sequences between -105 and -204 base pairs upstream from the initiation site of viral transcription are required for glucocorticoid stimulation. The postulated protein coded for by the viral LTR is not involved in the hormone regulation of transcription.

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References

  1. Varmus, H.E., Ringold, G.M. and Yamamoto, K.R. In: Glucocorticoid Hormone Action (Eds. J.D. Baxter and G.G. Rousseau), Springer, New York, 1979, pp. 253–278.

    Google Scholar 

  2. Ringold, G.M., Yamamoto, K.R., Bishop, J.M. and Varmus, H.E. Proc. Natl. Acad. Sci. USA 74: 2879–2883, 1977.

    Article  PubMed  CAS  Google Scholar 

  3. Young, H.A., Shin, T.Y., Scolnick, E.M. and Parks, W.P. J. Virol. 21: 139–146, 1977.

    PubMed  CAS  Google Scholar 

  4. Ucker, D.S., Ross, S.R. and Yamamoto, K.R. Cell 27: 257–266, 1981.

    Article  PubMed  CAS  Google Scholar 

  5. Groner, B., Hynes, N.E., Rahmsdorf, U. and Ponta, H. Nucleic Acids Res. 11: 4713–4725, 1983.

    Article  PubMed  CAS  Google Scholar 

  6. Ringold, G.M., Yamamoto, K.R., Tomkins, G.M., Bishop, J.M. and Varmus, H.E. Cell 6: 299–305, 1975.

    Article  PubMed  CAS  Google Scholar 

  7. Scolnick, E.M., Young, H.A. and Parks, W.P. Virology 69: 148–156, 1976.

    Article  PubMed  CAS  Google Scholar 

  8. Firzlaff, J. and Diggelmann, H., 1984 (manuscript in preparation).

    Google Scholar 

  9. Buetti, E. and Diggelmann, H. Cell 23: 335–345, 1981.

    Article  PubMed  CAS  Google Scholar 

  10. Owen, D. and Diggelmann, H. J. Virol. 45: 148–154, 1983.

    PubMed  CAS  Google Scholar 

  11. Fasel, N., Pearson, K., Buetti, E. and Diggelmann, H. EMBO J. 1: 3–7, 1982.

    PubMed  CAS  Google Scholar 

  12. Fasel, N., Buetti, E., Firzlaff, J., Pearson, K. and Diggelmann, H. Nucleic Acids Res. 11, 6943–6955, 1983.

    Article  PubMed  CAS  Google Scholar 

  13. Diggelmann, H., Vessaz, A.L. and Buetti, E. Virology 122: 332–341, 1982.

    Article  PubMed  CAS  Google Scholar 

  14. Berk, A.J. and Sharp, P.A. Cell 12, 721–732, 1977.

    Article  PubMed  CAS  Google Scholar 

  15. Weaver, R.F. and Weissmann, C. Nucleic Acids Res. 7: 1175–1193, 1979.

    Article  PubMed  CAS  Google Scholar 

  16. Buetti, E. and Diggelmann, H. EMBO J. 2: 1423–1429, 1983.

    PubMed  CAS  Google Scholar 

  17. Diggelmann, H., Buetti, E., Fasel, N., Owen, D. and Vessaz, A. In: Understanding Breast Cancer: Chemical and laboratory Concepts (Eds. M. Rich and P. Furmanski), Marcel Dekker, New York, in press.

    Google Scholar 

  18. Maxam, A. and Gilbert, W. In: Methods in Enzymology 65 (Eds. L. Grossman and K. Moldave), Academic Press, New York, 1980, pp. 499–560.

    Google Scholar 

  19. Sollner-Webb, B. and Reeder, R.H. Cell 18: 485–499, 1979.

    Article  PubMed  CAS  Google Scholar 

  20. Hynes, N.E., Kennedy, N., Rahmsdorf, U. and Groner, B. Proc. Natl. Acad. Sci. USA 78: 2038–2042, 1981.

    Article  PubMed  CAS  Google Scholar 

  21. Nusse, R., Asselbergs, F.A.M., Salden, M.H.L., Michalides, R.J.A.M. and Bloemendal, H. Virology 91: 106–115, 1978.

    Article  PubMed  CAS  Google Scholar 

  22. Dahl, H.H.M. and Dickson, C. J. Virol. 29: 1131–1141, 1979.

    CAS  Google Scholar 

  23. Dickson, C, and Atterwill, M. J. Virol. 35, 349–361, 1980.

    PubMed  CAS  Google Scholar 

  24. Groner, B., Hynes, N.E. and Diggelmann, H, J. Virol. 30: 417–420, 1979.

    PubMed  CAS  Google Scholar 

  25. Robertson, D.L. and Varmus, H.E, J. Virol. 30: 576–589, 1979.

    PubMed  CAS  Google Scholar 

  26. Sen, G.C., Smith, S.W., Marcus, S.L. and Sarkar, N.H. Proc. Nati. Acad. Sci. USA 76: 1736–1740, 1979.

    Article  CAS  Google Scholar 

  27. Donehower, L.A., Fleurdelys, B. and Hager, G.L. J, Virol. 45: 941–949, 1983.

    CAS  Google Scholar 

  28. Kennedy, N., Knedlitschek, G., Groner, B., Hynes, N.E., Herrlich, P., Michalides, R. and Van Ooyen, A.J.J, Nature 295: 622–624, 1982.

    Article  PubMed  CAS  Google Scholar 

  29. Dickson, C. and Peters, G. J. Virol. 37: 36–47, 1981.

    PubMed  CAS  Google Scholar 

  30. Wheeler, D.A., Butel, J.S., Medina, D., Cardiff, R.D. and Hager, G.L. J. Virol. 46: 42–49, 1983.

    PubMed  CAS  Google Scholar 

  31. Van Ooyen, A.J.J., Michalides, R.J.A.M. and Nusse, R, J. Virol. 46: 362–370, 1983.

    PubMed  Google Scholar 

  32. Redmond, S.M.S. and Dickson, C. EMBO J. 2: 125–131, 1983.

    PubMed  CAS  Google Scholar 

  33. Majors, J.E. and Varmus, H.E. J. Virol. 47: 495–504, 1983.

    PubMed  CAS  Google Scholar 

  34. Huang, A.L., Ostrowski, M.C., Berard, D. and Hager, G.L. Cell 27: 245–255, 1981.

    Article  PubMed  CAS  Google Scholar 

  35. Lee, F., Mulligan, R., Berg, P. and Ringold, G, Nature 294: 228–232, 1981.

    Article  PubMed  CAS  Google Scholar 

  36. Groner, B., Herrlich, P., Kennedy, N., Ponta, H., Rahmsdorf, U. and Hynes, N.E. J. Cell Biochem. 20: 349–357, 1982.

    Article  PubMed  CAS  Google Scholar 

  37. Hynes, N.E., Kennedy, N., Rahmsdorf, U. and Groner, B. Proc. Natl. Acad. Sci. USA 78: 2038–2042, 1981.

    Article  PubMed  Google Scholar 

  38. Banerji, J., Rusconi, S. and Schaffner, W. Cell 27: 299–308, 1981.

    Article  PubMed  CAS  Google Scholar 

  39. Hynes, N.E., Van Ooyen, A.J.J., Kennedy, N., Herrlich, P., Ponta, H. and Groner, B. Proc. Nat. Acad. Sci. USA 80: 3637–3641, 1983.

    Article  PubMed  CAS  Google Scholar 

  40. Majors, J. and Varmus, H.E. Proc. Natl. Acad. Sci USA 80: 5866–5870, 1983.

    Article  PubMed  CAS  Google Scholar 

  41. Payvar, F., Wrange, O., Carlstedt-Duke, J., Okret, S., Gustafsson, J.A. and Yamamoto, K.R. Proc. Natl. Acad. Sci. USA 78: 6628–6632, 1981.

    Article  PubMed  CAS  Google Scholar 

  42. Payvar, F., Firestone, G.L., Ross, S.R., Chandler, V.L., Wrange, O., Carlstedt-Duke, J., Gustafsson, J.A. and Yamamoto, K.R. J. Cell Biochem. 19: 241–247, 1982.

    Article  PubMed  CAS  Google Scholar 

  43. Pfahl, M. Cell 31: 475–482, 1982.

    Article  PubMed  CAS  Google Scholar 

  44. Govindan, M.V., Spiess, E. and Majors, J. Proc. Nat. Acad. Sci. USA 79: 5157–5161, 1982.

    Article  PubMed  CAS  Google Scholar 

  45. Geisse, S., Scheidereit, C, Westphal, H.M., Hynes, N.E., Groner, B. and Beato, M. EMBO J. 1: 1613–1619, 1982.

    PubMed  CAS  Google Scholar 

  46. Scheidereit, C, Geisse, S., Westphal, H.M. and Beato, M. Nature 304: 749–752, 1983.

    Article  PubMed  CAS  Google Scholar 

  47. Chandler, V.L., Maler, B.A. and Yamamoto, K.R, Cell 33: 489–499, 1983.

    Article  PubMed  CAS  Google Scholar 

  48. Parker, M. Nature 304: 687–688, 1983.

    Article  PubMed  CAS  Google Scholar 

  49. Khoury, G. and Gruss, P. Cell 33: 313–314, 1983.

    Article  PubMed  CAS  Google Scholar 

  50. Schmid, W., Scherer, G., Danesch, U., Zentgraf, H., Matthias, P., Strange, C.M., Röwekamp, W. and Schütz, G. EMBO J. 1: 1287–1293, 1982.

    PubMed  CAS  Google Scholar 

  51. Fasel, N. Ph.D. Thesis, University of Lausanne, 1983.

    Google Scholar 

  52. Barta, A., Richards, R.I., Baxter, J.D. and Shine, J. Proc. Natl. Acad. Sci. USA 78: 4867–4871, 1981.

    Article  PubMed  CAS  Google Scholar 

  53. Cochet, M., Chang, A.C.Y. and Cohen, S.N. Nature 297: 335–339, 1982.

    Article  PubMed  CAS  Google Scholar 

  54. Mirault, M.E., Southgate, R. and Del Wart, E. EMBO J. 1: 1279–1285, 1982.

    PubMed  CAS  Google Scholar 

  55. Ptashne, M., Jeffrey, A., Johnson, A.D., Maurer, R., Meyer, B.J., Pabo, CO., Roberts, T.M. and Sauer, R.T. Cell 19: 1–11, 1980.

    Article  PubMed  CAS  Google Scholar 

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Authors
  1. H. Diggelmann
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  2. E. Buetti
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  3. D. Owen
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  4. N. Fasel
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  5. J. Firzlaff
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  6. A. L. Vessaz
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Editor information

Editors and Affiliations

  1. The Hebrew University of Jerusalem, Israel

    Yechiel Becker

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© 1985 Martinus Nijhoff Publishing, Boston

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Diggelmann, H., Buetti, E., Owen, D., Fasel, N., Firzlaff, J., Vessaz, A.L. (1985). A Short Segment of Viral DNA is Required for the Stimulation of Transcription of Mouse Mammary Tumor Virus Genes by Glucocorticoid Hormones. In: Becker, Y., Hadar, J. (eds) Viral Messenger RNA. Developments in Molecular Virology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2585-7_16

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  • DOI: https://doi.org/10.1007/978-1-4613-2585-7_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9621-8

  • Online ISBN: 978-1-4613-2585-7

  • eBook Packages: Springer Book Archive

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