Recombinant DNA Technology and Characterization of Recombinant Alpha-2 Interferon
The strain of E. coli, KMAC-43, used for the large-scale production of alpha-2 interferon was engineered at Schering by Leibowitz and his co-workers. The alpha-2 interferon gene used in this construction was obtained from a cDNA clone, Hif-SN206, developed by Weissmann. The large-scale method developed in our laboratory for the purification of alpha-2 interferon from the bacterial extracts produces highly pure, crystalline alpha-2 interferon which is dissolved in 20 mM phosphate buffer at neutral pH to constitute bulk drug solution that is used in various formulations.
The protein has been thoroughly characterized using physico-chemical and biological methods. The average specific activity of clinical grade alpha-2 interferon is 1.7 × 108 u/mgP. The purity of the product has been established by reversed phase HPLC (>98% pure), amino acid analysis, amino acid sequencing, circular dichroism spectrometry, ultracentrifugation, SDS-PAGE and 2-D gel electrophoresis. Details of these methods and results will be discussed.
KeywordsAverage Specific Activity Cyanopropyl Silane
Unable to display preview. Download preview PDF.
- 1.Weissmann C, Nagata S, Boll W, Foutoulakis M, Fujisawa A, Fujisawa JI, Haynes J, Henco K, Mantei N, Ragg H, Schein C, Schmid J, Shaw G, Streuli M, Taira H, Todokoro K, Weidle U: Structure and expressions of human alpha-interferon genes. Abstr. ICN-UCLA Symposium on Chemistry and Biology of Interferons: Relationship to Therapeutics, 1982.Google Scholar
- 4.Weissmann C: Interferon 3. Academic Press, 1981, p. 102.Google Scholar
- 5.Nagabhushan TL, Bond M: unpublished results.Google Scholar
- 6.Leibowitz P, et al: U.S. Patent 4, 315, 852, 1982.Google Scholar
- 7.Nagabhushan TL, Le HV, Kosecki R, Reichert P, Sharma B, Trotta P: unpublished results.Google Scholar
- 12.Bond M: unpublished results.Google Scholar
- 14.Mihalyi E: Application of Proteolytic Enzymes to Protein Structure Studies, 2nd ed., Vol. I, 1978, p. 142.Google Scholar
- 15.Rose K, Priddle JD, Offord RE, Esnouf MP: J Biochem 187: 239–243, 1980.Google Scholar
- 26.Cell Tech Culture Product Division, Slough, UK.Google Scholar