Skip to main content

Utilization of λ Control Elements for Gene Expression Studies in Escherichia Coli

  • Chapter
Recombinant DNA Research and Viruses

Summary

A family of plasmids designed for studying gene expression in bacteria is described. The system employs λ regulatory elements. Transcription is regulated by the thermolabile cI repressor. Transcription termination signals can be introduced between the λ PL promoter and several structural genes. This system permits qualitative and quantitative studies of termination signals.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Rosenberg, M. and Court, D., Ann. Rev. Genet. 13: 319–353, 1979.

    Article  CAS  Google Scholar 

  2. Gold, L., Pribnow, D., Schneider T., Shinedling, S., Singer, B.S. and Stormo, G., Ann. Rev. Microbiol. 35: 3653–403, 1981.

    Article  Google Scholar 

  3. Gottesman, M., Oppenheim, A. and Court, D. Cell 29: 727–728, 1982.

    Article  PubMed  CAS  Google Scholar 

  4. Gottesman, S. Gottesman, M., Shaw, J. and Pearson, M., Cell 24:225–233, 1981.

    Article  PubMed  CAS  Google Scholar 

  5. Maniatis, T., Fritsch, E.F. and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1982.

    Google Scholar 

  6. Hedgpeth, J., Ballivet, M. and Eisen, H., Molec. gen. Genet., 163: 197–203, 1978.

    Article  PubMed  CAS  Google Scholar 

  7. Bernard, H., Remault, E., Hershfield, M., Das, H., Helinski, D., Yanofsky, C. and Franklin, N., Gene 5: 59–76, 1979.

    Article  PubMed  CAS  Google Scholar 

  8. Remaut, E., Stanssens, P. and Fiers, w., Gene 15: 81–93, 1981.

    Article  PubMed  CAS  Google Scholar 

  9. Derom, C., Gheysen, D. and Fiers, W., Gene 17: 45–54,1982.

    Article  PubMed  CAS  Google Scholar 

  10. Queen, C. J. Molec. Appl. Genet., 2: 1–10,1983.

    CAS  Google Scholar 

  11. Herskowitz, I. and Hagen, D., Ann Rev.Genet. 14: 399–445, 1980.

    Article  PubMed  CAS  Google Scholar 

  12. Hershey, A., (ed.), The Bacteriophage Lambda, Cold Spring Harbor Laboratory, Cold Spring Harbor, 1981.

    Google Scholar 

  13. Bolivar, F., Rodriguez, R.L., Greene, P.Y., Betlach, M.C., Heynecker, H.L., Boyer, H.W., Crosa, Y.H. and Falkow, S, Gene 2:95–113, 1977.

    Article  PubMed  CAS  Google Scholar 

  14. Casadaban, M., Chou, I. and Cohen, S., J. Bact. 143: 971–980, 1980.

    PubMed  CAS  Google Scholar 

  15. Close, T. and Rodriguez, R.L., Gene 20: 305–316, 1982.

    Article  PubMed  CAS  Google Scholar 

  16. McKinney, K., Shimatake, H., Court, D., Schmeissner, U., Brady, C. and Rosenberg, M., in J.G. Chirikjian and T.S. Papas, (eds.), Gene Amplification and Analysis, vol.2., Elsevier-North Holland, Amsterdam, 1981 pp. 383–415.

    Google Scholar 

  17. Honigman, A., et al, this volume.

    Google Scholar 

  18. Oppenheim, A.B., Katzir, N., Koby, S. and Locker-Giladi, H., this volume.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Martinus Nijhoff Publishing, Boston

About this chapter

Cite this chapter

Oppenheim, A.B. et al. (1985). Utilization of λ Control Elements for Gene Expression Studies in Escherichia Coli . In: Becker, Y., Hadar, J. (eds) Recombinant DNA Research and Viruses. Developments in Molecular Virology, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2565-9_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-2565-9_5

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9611-9

  • Online ISBN: 978-1-4613-2565-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics