Development of Plasmids and Cloning Procedures

  • Rudolf Eichenlaub
Part of the Developments in Molecular Virology book series (DMVI, volume 5)


The investigation of the F factor of Escherichia coli is chosen as an example for the recent developments in the field of plasmid research. F-derived mini-plasmids were constructed and their properties with respect to replication and copy number control were studied, applying numerous experimental approaches including electron microscopy, mutagenesis, cloning of subfragments, expression of gene products and DNA sequencing. Thus, insights into the mechanism of replication, copy number control and partitioning were achieved which finally allowed construction of special purpose cloning vectors based on plasmid F.


Plasmid Mutant BamHI Fragment Replicative Intermediate High Copy Number Plasmid Plasmid Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Konisky, J. In: The bacteria (Ed. L.C. Gunsalus) Vol. VI Academic Press, New York, 1978, pp. 71–136.Google Scholar
  2. 2.
    Willets, N. and Skurray, R. Ann. Rev. Genet. 14:41–76, 1980.CrossRefGoogle Scholar
  3. 3.
    Falkow, S. Infectious multiple drug resistance. Pion Limited, London, 1975.Google Scholar
  4. 4.
    Cohen, S.N. and Chang, A.C.Y. Proc. Nat. Acad. Sci. USA 70:1293–1297, 1973.PubMedCrossRefGoogle Scholar
  5. 5.
    Cohen, S.N., Chang, A.C.Y. and Hsu, L. Proc. Nat. Acad. Sci. USA 69:2110–2114, 1972.PubMedCrossRefGoogle Scholar
  6. 6.
    Cohen, S.N. and Chang, A.C.Y. Mol. Gen. Genet. 134:133–141, 1974.PubMedCrossRefGoogle Scholar
  7. 7.
    Staudenbauer, W.L. Curr. Top. Microbiol. Immunol. 83: 93–156, 1978.PubMedGoogle Scholar
  8. 8.
    Collins, J. Curr. Top. Microbiol. Immunol. 78:121–170, 1977.Google Scholar
  9. 9.
    Hershfield, V., Boyer, H.W., Yanofsky, C., Lovett, M.A. and Helinski, D.R. Proc. Nat. Acad. Sci. USA 71: 3455–3459, 1974.PubMedCrossRefGoogle Scholar
  10. 10.
    Clewell, D.B. J. Bacteriol. 1l0:667–676, 1972.Google Scholar
  11. 11.
    Hershfield, V., Boyer, H.W., Chow, L. and Helinski, D.R. J. Bacteriol. 126: 447–453, 1976.PubMedGoogle Scholar
  12. 12.
    Covey, C, Richardson, D. and Carbon, J. Mol. Gen. Genet. 145:155–158, 1976.PubMedCrossRefGoogle Scholar
  13. 13.
    Rodriguez, R.L., Bolivar, F., Goodman, H.W., Boyer, H.W. and Betlach, M. In: Molecular Mechanisms in the Control of Gene Expression (Eds. D.P. Nierlich, W.J. Rutter and C.F. Fox) Academic Press, New York, 1976, pp. 471–478.Google Scholar
  14. 14.
    Bolivar, F., Rodriguez, R.L., Greene, P.J., Betlach, M.C., Heyneker, H.L., Boyer, H.B. Crosa, J.H. and Falkow, S. Gene 2:95–113, 1977.PubMedCrossRefGoogle Scholar
  15. 15.
    Bolivar, F. Gene 4:121–136, 1978.PubMedCrossRefGoogle Scholar
  16. 16.
    Timmis, K.N. In: Genetics as a tool in Microbiology (Eds. S.W. Glover and D.A. Hopwood) Cambridge University Press, Cambridge, 1981, pp. 49–109.Google Scholar
  17. 17.
    Methods in Enzymology, Vol. 68 (Ed. R. Wu) Academic Press, New York, 1979.Google Scholar
  18. 18.
    Lane, H.E.D. Plasmid 5:100–126, 1981.PubMedCrossRefGoogle Scholar
  19. 19.
    Timmis, K., Cabello, F. and Cohen, S.N. Proc. Nat. Acad. Sci. USA 72:2242–2246, 1975.PubMedCrossRefGoogle Scholar
  20. 20.
    Lovett, M.A. and Helinski, D.R. J. Bacteriol. 127:982–989, 1976.PubMedGoogle Scholar
  21. 21.
    Skurray, R.A., Guyer, M.S., Timmis, K.N., Cabello, F., Cohen, S.N., Davidson, N. and Clark, A.J. J. Bacteriol. 127:1571–1575, 1976.PubMedGoogle Scholar
  22. 22.
    Guyer, M.S., Figurski, D. and Davidson, N. J. Bacteriol. 127:988–997, 1976.PubMedGoogle Scholar
  23. 23.
    Palchandhuri, S. and Maas, W.K. Proc. Nat. Acad. Sci. USA 74:1190–1194, 1977.CrossRefGoogle Scholar
  24. 24.
    Maas, R. Proc. Nat. Acad. Sci. USA 50:1051–1055, 1963.PubMedCrossRefGoogle Scholar
  25. 25.
    Datta, N. In: Plasmids of Medical, Environmental and Commercial Importance (Eds. K.N. Timmis and A. Pünier) Elsevier/ North-Holland, Biomedical Press, Amsterdam, 1979, pp. 3–12.Google Scholar
  26. 26.
    Andres, J., Slocombe, P.M., Cabello, F., Timmis, J.K., Lurz, R., Burkhardt, H.-J. and Timmis, K.N. Mol. Gen. Genet. 168:1–25, 1979.PubMedCrossRefGoogle Scholar
  27. 27.
    Kollek, R., Oertel, W. and Goebel, W. Mol. Gen. Genet. 162:51–58, 1978.PubMedCrossRefGoogle Scholar
  28. 28.
    Crosa, J.H., Luttropp, L.K. and Falkow, S. Cold Spring Harb. Symp. Quant. Biol. 43:111–120, 1978.Google Scholar
  29. 29.
    Kolter, R. and Helinski, D.R. Plasmid 1:571–580, 1978.PubMedCrossRefGoogle Scholar
  30. 30.
    Kahn, M.L., Figurski, D., Ito, L. and Helinski, D.R. Cold Spring Harb. Symp. Quant. Biol. 43:99–103, 1979.PubMedGoogle Scholar
  31. 31.
    Wehlmann, H. and Eichenlaub, R. Molec. Gen. Genet. 180:205–211, 1980.PubMedCrossRefGoogle Scholar
  32. 32.
    Figurski, D., Kolter, R., Meyer, R., Kahn, M., Eichenlaub, R. and Helinski, D.R. In: Microbiology — 1978 (Ed. D. Schlessinger) Am. Soc. Microbiol., Washington D.C., 1978, pp. 105–109.Google Scholar
  33. 33.
    Manis, J.J. and Kline, B.C. Mol. Gen. Genet. 152:175–182, 1977.PubMedCrossRefGoogle Scholar
  34. 34.
    Lovett, M.A., Sparks, R.B. and Helinski, D.R. Proc. Nat. Acad. Sci. USA 72:2905–2909, 1975.PubMedCrossRefGoogle Scholar
  35. 35.
    Cabello, F., Timmis, K. and Cohen, S.N. Nature 259:285–290, 1976.PubMedCrossRefGoogle Scholar
  36. 36.
    Lovett, M.A., Katz, L. and Helinski, D.R. Nature 251:337–340, 1974.PubMedCrossRefGoogle Scholar
  37. 37.
    Eichenlaub, R., Sparks, R.B. and Helinski, D.R. J. Bacteriol. 138:257–260, 1979.PubMedGoogle Scholar
  38. 38.
    Sparks, R.B. and Helinski, D.R. Nature 277:572–575, 1979.PubMedCrossRefGoogle Scholar
  39. 39.
    Guerry, P., Le Blanc, D.J. and Falkow, S. J. Bacteriol. 116:1064–1066, 1973.PubMedGoogle Scholar
  40. 40.
    Hirt, B. J. Mol. Biol. 26:365–369, 1967.CrossRefGoogle Scholar
  41. 41.
    Eichenlaub, R., Figurski, D. and Helinski, D.R. Proc. Nat. Acad. Sci. USA 74:1138–1141, 1977.PubMedCrossRefGoogle Scholar
  42. 42.
    Eichenlaub, R., Wehlmann, H. and Ebbers, J. In: Molecular Biology, Pathogenicity and Ecology of Bacterial Plasmids (Eds. S. Levy, E. Koenig and R. Clowes) Plenum Press, New York, 1981, pp. 327–336.Google Scholar
  43. 43.
    Zubay, G. Ann. Rev. Genet. 7:267–287, 1973.PubMedCrossRefGoogle Scholar
  44. 44.
    Frazer, A.C. and Cortiss III, R. Curr. Top. Microbiol. Immunol. 69:1–84, 1975.PubMedGoogle Scholar
  45. 45.
    Eichenlaub, R. J. Bacteriol. 120:1476–1477, 1974.PubMedGoogle Scholar
  46. 46.
    Yang, H.-L., Zubay, G. and Levy, S.B. Proc. Nat. Acad. Sci. USA 73:1509–1512, 1976.PubMedCrossRefGoogle Scholar
  47. 47.
    Kennedy, N., Bentin, L., Achtman, M., Skurray, R., Rahmsdorf, U. and Herrlich, P. Nature 270:580–585, 1977.PubMedCrossRefGoogle Scholar
  48. 48.
    Eichenlaub, R. and Wehlmann, H. Mol. Gen. Genet. 180:201–204, 1980.CrossRefGoogle Scholar
  49. 49.
    Komai, N., Nishizawa, T., Hayakawa, Y., Murotsu, T. and Matsubara, K. Mol. Gen. Genet. 186:193–203, 1982.PubMedCrossRefGoogle Scholar
  50. 50.
    Brack, C. Proc. Nat. Acad. Sci. USA 76:3164–3168, 1979.PubMedCrossRefGoogle Scholar
  51. 51.
    Wehlmann, H. and Eichenlaub, R. Plasmid 5:259–266, 1981.PubMedCrossRefGoogle Scholar
  52. 52.
    Murotsu, T., Matsubara, K., Sugisaki, H. and Takanami, M. M. Gene 15:257–271, 1981.PubMedCrossRefGoogle Scholar
  53. 53.
    Tolun, A. and Helinski, D.R. Cell 24:687–694, 1981.PubMedCrossRefGoogle Scholar
  54. 54.
    Tolun, A. and Helinski, D.R. Mol. Gen. Genet. 186:372–377, 1982.PubMedCrossRefGoogle Scholar
  55. 55.
    Kline, B.C. and Palchandhuri, S. Plasmid 4:281–291, 1980.PubMedCrossRefGoogle Scholar
  56. 56.
    Kline, B.C. and Lane, D. Plasmid 4:231–232, 1980.PubMedCrossRefGoogle Scholar
  57. 57.
    Gardner, R.C., Malcolm, L., Bergquist, P.L. and Lane, H.E.D. Mol. Gen. Genet. 188, 345–352, 1982.PubMedCrossRefGoogle Scholar
  58. 58.
    Ogura, T. and Hiraga, S. Cell 32:351–360, 1983.PubMedCrossRefGoogle Scholar
  59. 59.
    Ogura, T., Miki, T. and Hiraga S. Proc. Nat. Acad. Sci. USA 77:3993–3997, 1980.PubMedCrossRefGoogle Scholar
  60. 60.
    Jacob, F., Brenner, S. and Cuzin, F. Cold Spring Harb. Symp. Quant. Biol. 28:329–348, 1963.Google Scholar
  61. 61.
    Cuzin, F. and Jacob, F. Ann. Inst. Pasteur, Paris, 112:398–418, 1967.Google Scholar
  62. 62.
    Humphreys, G.O., Willshaw, G.A., Smith, H.R. and Anderson, E.S. Mol. Gen. Genet. 145:101–108, 1976.PubMedCrossRefGoogle Scholar
  63. 63.
    Freese, E., Bautz, E. and Bautz-Freese, E. Proc. Nat. Acad. Sci. USA 47:845–855, 1961.PubMedCrossRefGoogle Scholar
  64. 64.
    Eichenlaub, R. J. Bacteriol. 138:559–566, 1979.PubMedGoogle Scholar
  65. 65.
    Collins, J. and Brünning, H.J. Gene 4:85–107, 1978.PubMedCrossRefGoogle Scholar
  66. 66.
    Hohn, B. and Murray, K. Proc. Nat. Acad. Sci. USA 74:3259–3265, 1977.PubMedCrossRefGoogle Scholar
  67. 67.
    Shortle, D., DiMaio, D. and Nathans, D. Ann. Rev. Genet. 15:265–294, 1981.PubMedCrossRefGoogle Scholar
  68. 68.
    Nordström, K. Plasmid 9:1–7, 1983.PubMedCrossRefGoogle Scholar
  69. 69.
    Tsurimoto, T. and Matsubara, K. In: “ICN-UCLA Symp. on Mol. and Cellular Biol.” Vol. 22, 1981, pp. 263–275.Google Scholar
  70. 70.
    Ebbers, J. and Eichenlaub, R. J. Bacteriol. 147:736–743, 1981.PubMedGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1985

Authors and Affiliations

  • Rudolf Eichenlaub
    • 1
  1. 1.Lehrstuhl Biologie der MikroorganismenRuhr-Universität BochumBochum 1Federal Republic of Germany

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