Abstract
Bovine leukemia virus (BLV) is an exogenous retrovirus which induces lymphoid proliferation (persistent lymphocytosis and/or lymphosarcoma) in cattle (1). Understanding the phenomenon of cell transformation at the molecular level will depend on the availability of specific probes. Among these, a cloned provirus is a mandatory requirement to investigate the presence of a cellular oncogene in the BLV genome and to determine the complete nucleotide sequence of a BLV provirus. For this reason, a BLV provirus having SacI restriction sites only in the long terminal repeat (LTR) regions was chosen for the molecular cloning procedure described below.
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References
Burny, A. 1984. In: Mechanisms of Viral Leukaemogenesis (Eds. J.M. Goldman and O. Jarrett), Churchill Livingstone, London (in press).
Leder, P., Tiemeier, D. and Enquist, L. 1977. Science 196:175–177.
Maniatis, T., Hardison, R.D., Lacy, E., Lauer, J., O’Connell, C., Quon, D., Sim, G.K. and Efstratiadis, A. 1978. Cell 15:687–701.
Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning: A Laboratory Mannual. Cold Spring Harbor Laboratory, U.S.A.
Blattner, F.R., Williams, B.G., Blechl, A.E., Denniston-Thompson, K., Faber, H.E., Furlong, L.A., Grunwald, D.J., Kiefer, D.O., Moore, D.D., Sheldon, E.L. and Smithies, O. 1977. Science 196:161–169.
Deschamps, J., Kettmann, R. and Burny, A. 1981. J. Virol. 40:605–609.
Bruck, C., Portetelle, D., Burny, A. and Zavada, J. 1982. Virology 122:353–362.
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© 1985 Martinus Nijhoff Publishing, Boston
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Deschamps, J., Burny, A., Kettmann, R. (1985). Cloning of Bovine Leukemia Virus Proviral Information. In: Becker, Y., Hadar, J. (eds) Recombinant DNA Research and Viruses. Developments in Molecular Virology, vol 5. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2565-9_10
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DOI: https://doi.org/10.1007/978-1-4613-2565-9_10
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