Abstract
The standard system for the study of antigen-specific, thymus dependent, antibody responses utilizes mouse spleen. B and T lymphocytes (Sephadex G10 nonadherent spleen) are cultured with accessory cells (plastic adherent spleen) and antigen (red cells or TNP/DNP-KLH) for 4-5 days to generate specific IgM PFC. The accessory cells likely induce functioning helper T cells, and the latter cooperate with B lymphocytes during antibody formation.
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Abbreviations
- DC:
-
dendritic cell
- PFC:
-
plaque forming cell
- KLH:
-
keyhole limpet hemocyanin
- MLR:
-
mixed leukocyte reaction
- MHC:
-
major histocompatibility complex
- DNP/TNP:
-
di/trinitrophenyl.
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© 1985 Plenum Press, New York
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Inaba, K., Steinman, R.M. (1985). Antibody Responses to T-Dependent Antigens: Contributions of Dendritic Cells and Helper T Lymphocytes. In: Klaus, G.G.B. (eds) Microenvironments in the Lymphoid System. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2463-8_44
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DOI: https://doi.org/10.1007/978-1-4613-2463-8_44
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