Antibody Responses to T-Dependent Antigens: Contributions of Dendritic Cells and Helper T Lymphocytes

  • Kayo Inaba
  • Ralph M. Steinman

Abstract

The standard system for the study of antigen-specific, thymus dependent, antibody responses utilizes mouse spleen. B and T lymphocytes (Sephadex G10 nonadherent spleen) are cultured with accessory cells (plastic adherent spleen) and antigen (red cells or TNP/DNP-KLH) for 4-5 days to generate specific IgM PFC. The accessory cells likely induce functioning helper T cells, and the latter cooperate with B lymphocytes during antibody formation.

Keywords

Nylon Allo Mitomycin 

Abbreviations

DC

dendritic cell

PFC

plaque forming cell

KLH

keyhole limpet hemocyanin

MLR

mixed leukocyte reaction

MHC

major histocompatibility complex

DNP/TNP

di/trinitrophenyl.

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Kayo Inaba
    • 1
  • Ralph M. Steinman
    • 1
  1. 1.The Rockefeller University and Irvington House InstituteNew YorkUSA

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