Antibody Responses to T-Dependent Antigens: Contributions of Dendritic Cells and Helper T Lymphocytes
The standard system for the study of antigen-specific, thymus dependent, antibody responses utilizes mouse spleen. B and T lymphocytes (Sephadex G10 nonadherent spleen) are cultured with accessory cells (plastic adherent spleen) and antigen (red cells or TNP/DNP-KLH) for 4-5 days to generate specific IgM PFC. The accessory cells likely induce functioning helper T cells, and the latter cooperate with B lymphocytes during antibody formation.
KeywordsDendritic Cell Antibody Response Helper Cell Accessory Cell Peripheral Lymphoid Organ
plaque forming cell
keyhole limpet hemocyanin
mixed leukocyte reaction
major histocompatibility complex
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- 8.Ashwell J., DeFranco A., Paul W., and Schwartz R. 1984. Antigen presentation by resting B cells: radiosensitivity of the antigen presentation function and two distinct pathways of T cell activation. J. Exp. Med. 159: 881–905.Google Scholar
- 10.Finkelman F.D. and Vitetta E.S. 1984. Role of surface immunoglobulin in B lymphocyte activation. Fed. Proc. 43: 2624- 2632.Google Scholar
- 11.Inaba K., Granelli-Piperno A., and Steinman R.M. 1983. Dendritic cells induce T lymphocytes to release B cell stimulating factors by an interleukin 2 dependent mechanism. J. Exp. Med. 158: 2040-2057.Google Scholar
- 12.Roehm N.W., Marrack P., and Kappler J.W. 1982. Antigen specific H-2 restricted helper T cell hybridomas. J. Exp. Med. 156: 191-204.Google Scholar
- 13.Inaba K., Witmer M.D., and Steinman R.M. 1984. The clustering of dendritic cells, helper T lymphocytes, and histocompatible B cells during primary antibody responses in vitro. Exp. Med. In press.Google Scholar
- 14.Inaba K., and Steinman R.M. 1984. Resting and sensitized T lymphocytes exhibit distinct stimulatory requirements for growth and lymphokine releases. J. Exp. Med. In Press.Google Scholar