Mouse Lymph Node Follicular Dendritic Cells: Quantitative Analysis and Isolation
Follicular dendritic cells (FDC) are peculiar non-lymphoid cells only present in lymph follicles especially in germinal centers. They retain antigens in form of immune complexes on the surface of their membranes (1, 2, 3). Few data are available concerning their nature, origin and function. Their study is difficult as they represent only a low percentage of the cell population inside the germinal centers. Recently we achieved the isolation of FDC from human tonsils and adenoids (4). After isolation human FDC form spherical structures where they envelope lymphoid cells (mainly B cells, some T helper cells) with their cytoplasmic extensions. Isolated tonsillar FDC bear IgM, IgA, IgG and IGE but no IgD on their surface. They share also some antigens with macrophages (HLA-DR and Leu-M3) and express, at high density, C3b and Fc receptors (5).
KeywordsAlbumin Leukemia Citrate Sedimentation Germinal
bovine serum albumin
follicular dendritic cell
goat anti-mouse immunoglobulins bound to 20 nm gold particles
phosphate buffer solution
sheep red blood cells
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