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Mouse Lymph Node Follicular Dendritic Cells: Quantitative Analysis and Isolation

  • Ernst Heinen
  • Cécile Kinet-Denoël
  • Daniel Radoux
  • Léon Simar

Abstract

Follicular dendritic cells (FDC) are peculiar non-lymphoid cells only present in lymph follicles especially in germinal centers. They retain antigens in form of immune complexes on the surface of their membranes (1, 2, 3). Few data are available concerning their nature, origin and function. Their study is difficult as they represent only a low percentage of the cell population inside the germinal centers. Recently we achieved the isolation of FDC from human tonsils and adenoids (4). After isolation human FDC form spherical structures where they envelope lymphoid cells (mainly B cells, some T helper cells) with their cytoplasmic extensions. Isolated tonsillar FDC bear IgM, IgA, IgG and IGE but no IgD on their surface. They share also some antigens with macrophages (HLA-DR and Leu-M3) and express, at high density, C3b and Fc receptors (5).

Keywords

Gold Particle Lymphoid Cell Germinal Center Mouse Lymph Node Follicular Dendritic Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BSA

bovine serum albumin

FDC

follicular dendritic cell

GAM-G20

goat anti-mouse immunoglobulins bound to 20 nm gold particles

PBS

phosphate buffer solution

SRBC

sheep red blood cells

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Ernst Heinen
    • 1
  • Cécile Kinet-Denoël
    • 1
  • Daniel Radoux
    • 1
  • Léon Simar
    • 1
  1. 1.Institute of Human HistologyUniversity of LiègeLiègeBelgium

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