Skip to main content
SpringerLink
Log in

Pre-B Cells in Rat Bone Marrow: Identification, Surface Markers and Isolation

  • Chapter
Microenvironments in the Lymphoid System

  • 29 Accesses

Abstract

In adult rats, as in other species, bone marrow has the highest potential for B-lymphocyte genesis as determined by a long-term repopulation assay for B-lymphocyte stem cells (1). These cells carry the Thy-1 and W3/13 antigens (2, 3). Pre-B cells characterized by the presence of µ heavy chains in the cytoplasm (cµ) but not on the cell surface (sµ) have been described in mice (4), men (5) and rabbits (6) but not in rats, and appear to be the immediate precursors of B-lymphocytes (7–10). The relation between pre-B cells and B-lymphocyte stem cells is not clear, however. Therefore, the aims of the present study were to identify cµ+sµ- pre-B cells in rat bone marrow and to find surface markers that would permit isolation of viable pre-B cells to subsequently assay their functional potential in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. SV. Hunt, In: “Immunobiology of Bone Marrow Transplantation”, S. Thierfelder, H. Rodand HJ. Kolb, eds., Springer Verlag, Berlin (1980) pp 193 – 203.

    Google Scholar 

  2. S.V. Hunt. Eur. J. Immunol. 9: 853 (1979).

    Article  PubMed  CAS  Google Scholar 

  3. M.J.S. Dyer and S.V. Hunt. J. Exp. Med. 154: 1164 (1981)

    Article  PubMed  CAS  Google Scholar 

  4. M.C. Raff, M. Megson, J.J.T. Owen and M.D. Cooper. Nature 259: 224 (1976).

    Article  PubMed  CAS  Google Scholar 

  5. W.E. Gathings, A.R. Lawton and M.D. Cooper. Eur. J. Immunol. 7: 804 (1977)

    Article  PubMed  CAS  Google Scholar 

  6. A.R. Hayward, M.A. Simons, A.R. Lawton, R.G. Mage and M.D. Cooper. J. Exp. Med. 148: 1367 (1978).

    Article  PubMed  CAS  Google Scholar 

  7. K.S. Landreth, C. Rosse and J. Clagett. J. Immunol. 127: 2027 (1981).

    PubMed  CAS  Google Scholar 

  8. R.L. Coffman. Immunol. Rev. 69: 5 (1983)

    Article  CAS  Google Scholar 

  9. D. Opstelten and D.G. Osmond. J. Immunol. 131: 2635 (1983).

    PubMed  CAS  Google Scholar 

  10. D.G. Osmond, F. Melchers and C.J. Paige. J. Immunol. 133: 86 (1984).

    PubMed  CAS  Google Scholar 

  11. A.F. Williams. Eur. J. Immunol. 5: 883 (1975).

    Article  CAS  Google Scholar 

  12. G.P. Spickett, M.R. Brandon, D.W. Mason, A.F. Williams and G.R. Woollett. J. Exp. Med. 158: 795 (1983).

    Article  PubMed  CAS  Google Scholar 

  13. F.G.M. Kroese, D. Opstelten, A.S. Wubbena, G.J. Deenen, E.H. Schwander, L. de Ley and P. Nieuwenhuis. This volume.

    Google Scholar 

  14. J. Rozing, P. Joling, P. Nieuwenhuis and L.M.B. Vaessen. This volume.

    Google Scholar 

  15. SV. Hunt . In: “Handbook of Experimental Immunology”. DM. Weir, ed., Blackwell, Oxford. Edition 1984, in press.

    Google Scholar 

  16. M. v.d. Giessen, S. Postma and T.H. The. Submitted, to Scand. J. Immunol.

    Google Scholar 

  17. S.V. Hunt, D.W. Mason and A.F. Williams. Eur. J. Immunol. 7: 817 (1977).

    Article  PubMed  CAS  Google Scholar 

  18. D.L. Greiner, I. Goldschneider and R.W. Barton. J. Exp. Med. 156: 1448 (1982).

    Article  PubMed  CAS  Google Scholar 

  19. I.D. Goldschneider, T.v Metcalf, T. Mandel and F.J. Bollum. J. Exp. Med. 152: 438 (1980)

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dept. of Histology, State University, Oostersingel 69/1, Groningen, The Netherlands

    D. Opstelten, G. J. Deenen & T. Jaarsma

  2. Dept. of Internal Medicine I, Erasmus University Rotterdam, The Netherlands

    J. Rozing

  3. Sir William Dunn School of Pathology, Oxford University, Oxford, UK

    S. V. Hunt

Authors
  1. D. Opstelten
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. J. Deenen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Jaarsma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Rozing
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. V. Hunt
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Nationanl Institute for Medical Research, London, England

    G. G. B. Klaus

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Plenum Press, New York

About this chapter

Cite this chapter

Opstelten, D., Deenen, G.J., Jaarsma, T., Rozing, J., Hunt, S.V. (1985). Pre-B Cells in Rat Bone Marrow: Identification, Surface Markers and Isolation. In: Klaus, G.G.B. (eds) Microenvironments in the Lymphoid System. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2463-8_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-2463-8_2

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9495-5

  • Online ISBN: 978-1-4613-2463-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation