Mutation Induction by Excess Deoxyribonucleotides in Saccharomyces Cerevisiae
Excess dTMP is toxic and mutagenic with exponentially growing dTMP efficient uptaking yeast strains 831 rho+ and 833 rho. The respiratory deficient strain 833 exhibits a tenfold sensitivity to the genotoxicity of excess dTMP. Mutant yield in the forward mutation system CAN1 → canl after dTMP excess is comparable to that found after irradiation with UV254nm. Excess dTMP is a poor mutagen in stationary phase cells of both strains. Mutagenicity of excess dTMP is not found in an ochre mutant allele (ade2-l). Exposure of exponentially growing cells to other deoxyribonucleotides (dCMP, dAMP, and dGMP) reveal these nucleotides to have mutagenic potential as well.
KeywordsYeast Strain Mutation Induction Stationary Phase Cell Nucleic Acid Metabolism Mutant Yield
Unable to display preview. Download preview PDF.
- 7.W. W. Fäth, and M. Brendel, Isolation and properties of yeast mutants with highly efficient thymidylate utilization, Z. Naturforsch., 31c:468–478 (1976).Google Scholar