Mutation Induction by Excess Deoxyribonucleotides in Saccharomyces Cerevisiae

  • Martin Brendel
Part of the Basic Life Sciences book series (BLSC, volume 31)


Excess dTMP is toxic and mutagenic with exponentially growing dTMP efficient uptaking yeast strains 831 rho+ and 833 rho. The respiratory deficient strain 833 exhibits a tenfold sensitivity to the genotoxicity of excess dTMP. Mutant yield in the forward mutation system CAN1canl after dTMP excess is comparable to that found after irradiation with UV254nm. Excess dTMP is a poor mutagen in stationary phase cells of both strains. Mutagenicity of excess dTMP is not found in an ochre mutant allele (ade2-l). Exposure of exponentially growing cells to other deoxyribonucleotides (dCMP, dAMP, and dGMP) reveal these nucleotides to have mutagenic potential as well.


Yeast Strain Mutation Induction Stationary Phase Cell Nucleic Acid Metabolism Mutant Yield 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Martin Brendel
    • 1
  1. 1.Institut für MikrobiologieJ. W. Goethe-UniversitätFrankfurt/MainGermany

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