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2-Aminopurine Mutagenesis in Escherichia Coli

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Genetic Consequences of Nucleotide Pool Imbalance

Part of the book series: Basic Life Sciences ((BLSC,volume 31))

Abstract

Base analog mutagens are structurally modified bases which sufficiently mimic the natural bases to be incorporated into DNA but whose specificity of incorporation and subsequent templating properties lead to mutation [17, 19]. Two base analogs are quite predominant in mutational literature, the pyrimidine analog 5-bromo-uracil (5BU) and the purine analog 2-aminopurine (2AP). While this study does draw from our knowledge of 5BU-induced mutagenesis, our experimental efforts have concentrated on 2-aminopurine and some related purine analogs. The chemical and biological properties of 2AP have recently been reviewed by Ronen (see Ref. 49).

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  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, 27709, USA

    Barry W. Glickman

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  1. National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

    Frederick J. de Serres

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© 1985 Plenum Press, New York

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Glickman, B.W. (1985). 2-Aminopurine Mutagenesis in Escherichia Coli . In: de Serres, F.J. (eds) Genetic Consequences of Nucleotide Pool Imbalance. Basic Life Sciences, vol 31. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2449-2_22

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  • DOI: https://doi.org/10.1007/978-1-4613-2449-2_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9488-7

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