Induction of Mutation and Chromosome Damage by Excess Bases and Nucleosides

  • Diana Anderson
Part of the Basic Life Sciences book series (BLSC, volume 31)

Summary

The studies reported in this communication were conducted to determine if naturally occurring cell constituents could themselves cause mutation. All the bases and their corresponding nucleosides have been shown in culture to produce chromosome damage in P388 mouse lymphoma and Chinese hamster ovary cells (CHO) and adenosine has produced an increased incidence of sister chromatid exchanges in CHO cells. In addition thymidine has produced an increase in V79 cells resistant to 8-azaguanine and ouabain, although no effects were seen in six strains of Salmonella typhimurium. Such damage probably arises as a result of imbalanced DNA-precursor pools. Thus mutagenic events may arise by mechanisms unrelated to direct alterations of DNA.

Keywords

Adenosine Myeloma Pyrene Adenine Pyrimidine 

Abbreviations

dCTP

deoxycytidine triphosphate

dTTP

deoxythymidine triphosphate

BUdR

5-bromo-2-deoxyuridine

PBS

phosphate-buffered saline

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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Diana Anderson
    • 1
  1. 1.The British Industrial Biological Research AssociationSurreyUK

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