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Studies on the Transposition of IS1

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Plasmids in Bacteria

Abstract

The insertion sequence, IS1, is among the smallest insertion elements known. It is a member of a class of elements which includes IS10, IS50, and IS903 which are frequently found in pairs flanking a nontransposable DNA segment. In concert, the 2 flanking elements are able to promote transposition of the entire structure. This type of transposable element has been called a compound or composite transposon. The best characterized IS1-flanked compound transposon is perhaps Tn9 in which 2 directly repeated copies of IS1 flank a 1.1-kb segment of DNA which encodes chloramphenicol acetyltransferase (CAT) (1). Unlike most other elements of this class, however, IS1 is present in the chromosomes of a variety of enterobacteria, in some cases in many copies (2).

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Author information

Authors and Affiliations

  1. Centre de Recherche de Biochimie et Génétique Cellulaire du C.N.R.S., 118, Route de Narbonne, 31062, Toulouse Cedex, France

    Michael Chandler

  2. Molecular Biology University of Southern California, 90089-1481, Los Angeles, California, USA

    David J. Galas

Authors
  1. Michael Chandler
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  2. David J. Galas
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Editor information

Editors and Affiliations

  1. University of California, San Diego, La Jolla, California, USA

    Donald R. Helinski

  2. Stanford University, Stanford, California, USA

    Stanley N. Cohen

  3. University of Michigan, Ann Harbor, Michigan, USA

    Don B. Clewell

  4. Genex Corporation, Gaithersburg, Maryland, USA

    David A. Jackson

  5. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1985 Plenum Press, New York

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Chandler, M., Galas, D.J. (1985). Studies on the Transposition of IS1. In: Helinski, D.R., Cohen, S.N., Clewell, D.B., Jackson, D.A., Hollaender, A. (eds) Plasmids in Bacteria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2447-8_7

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  • DOI: https://doi.org/10.1007/978-1-4613-2447-8_7

  • Publisher Name: Springer, Boston, MA

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  • Online ISBN: 978-1-4613-2447-8

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