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Linear Plasmids with Terminal Inverted Repeats Obtained from Streptomyces Rochei and Kluyveromyces Lactis

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Plasmids in Bacteria

Summary

Linear plasmids with inverted terminal repeats of 614 bp were obtained from Streptomyces rochei which produced lankacidin. The 5’ ends were blocked by the association of a terminal protein, A DNA model of racket frame-like structure is presented implying the juxtaposition of 2 double-stranded DNAs of the same sequence through the binding of cohesive proteins which recognize and bind to the DNA.

Two linear plasmids with the inverted terminal repeats of 202 and 184 bp were obtained from a yeast, Kluyveromyces lactis. A killer toxin was produced from the shorter plasmid. The protein toxin inhibited the adenylatecyclase activity of the yeast membrane.

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Author information

Authors and Affiliations

  1. Mitsubishi-Kasei Institute of Life Sciences, Minamiooya 11, Machida, Tokyo, Japan, 194

    Kenji Sakaguchi, Hirohiko Hirochika & Norio Gunge

Authors
  1. Kenji Sakaguchi
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  2. Hirohiko Hirochika
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  3. Norio Gunge
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Editor information

Editors and Affiliations

  1. University of California, San Diego, La Jolla, California, USA

    Donald R. Helinski

  2. Stanford University, Stanford, California, USA

    Stanley N. Cohen

  3. University of Michigan, Ann Harbor, Michigan, USA

    Don B. Clewell

  4. Genex Corporation, Gaithersburg, Maryland, USA

    David A. Jackson

  5. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1985 Plenum Press, New York

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Sakaguchi, K., Hirochika, H., Gunge, N. (1985). Linear Plasmids with Terminal Inverted Repeats Obtained from Streptomyces Rochei and Kluyveromyces Lactis . In: Helinski, D.R., Cohen, S.N., Clewell, D.B., Jackson, D.A., Hollaender, A. (eds) Plasmids in Bacteria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2447-8_32

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  • DOI: https://doi.org/10.1007/978-1-4613-2447-8_32

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9487-0

  • Online ISBN: 978-1-4613-2447-8

  • eBook Packages: Springer Book Archive

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